Determination of antifungal activity of polyenzyme preparation Candidaseb using in vitro methods.

Abstract

A new polyenzyme formulation 'Candidaseb' was tested for its ability to inhibit clinically important fungi. After its antifungal activity was established, an assay was standardized to determine whether any potentiation effect was possible if Candidaseb was used in conjunction with three standard antifungal agents viz, Amphotericin B, Miconazole and Ketoconazole against 5 clinically significant fungi viz., Candida albicans, Cryptococcus neoformans, Aspergillusniger, Microsporum gypseum and Trichophyton rubrum. A synergistic effect could be established when a combination of Candidaseb and antifungal was used against all 5 pathogenic fungi. A reduction in MIC values of standard drugs could be observed. Also, Miconazole, which had proved ineffective against Candida and Cryptococcus became effective when it was used in combination with Candidaseb indicating a potentiating effect on the drug.