Preparation and characterization of polyurethane/chitosan/CNT nanofibrous scaffold for cardiac tissue engineering

IF 4.4 2区化学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

ACS Applied Polymer Materials Pub Date : 2021-06-01 DOI:10.1016/j.ijbiomac.2021.03.001

Parisa Ahmadi , Niloofar Nazeri , Mohammad Ali Derakhshan , Hossein Ghanbari

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引用次数: 55

Abstract

Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide. Since heart has very limited regeneration capacity, cardiac tissue engineering (TE) to produce a bioactive scaffold is considered. In this study, a series of polyurethane solutions (5–7%wt) in aqueous acetic acid were prepared using electrospinning. A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM). Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay. The biocompatibility of electrospun random and aligned nanofibrous scaffolds with H9C2 Cells was confirmed. The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.

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心脏组织工程用聚氨酯/壳聚糖/碳纳米管纳米纤维支架的制备与表征

心肌细胞心肌梗死是世界范围内心力衰竭(HF)的主要原因。由于心脏的再生能力非常有限，因此考虑利用心脏组织工程(TE)来制造具有生物活性的支架。本研究采用静电纺丝法在醋酸水溶液中制备了一系列5-7%wt的聚氨酯溶液。制备了多种取向随机排列的聚氨酯/壳聚糖/碳纳米管复合纳米纤维支架，以模拟细胞外基质(ECM)。然后利用场发射扫描电镜(FESEM)、透射电镜(TEM)、x射线衍射(XRD)、水接触角、降解研究、拉伸测试、电阻测量和细胞活力测定对静电纺纳米纤维进行了表征。证实了静电纺丝随机排列纳米纤维支架与H9C2细胞的生物相容性。结果表明，制备的PU/Cs/CNT复合纳米纤维支架具有良好的导电性，排列纳米纤维是一种具有纳米级特征的梗死心肌再生支架材料。

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来源期刊

ACS Applied Polymer Materials Multiple-

CiteScore

7.20

自引率

6.00%

发文量

810

期刊介绍： ACS Applied Polymer Materials is an interdisciplinary journal publishing original research covering all aspects of engineering, chemistry, physics, and biology relevant to applications of polymers. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates fundamental knowledge in the areas of materials, engineering, physics, bioscience, polymer science and chemistry into important polymer applications. The journal is specifically interested in work that addresses relationships among structure, processing, morphology, chemistry, properties, and function as well as work that provide insights into mechanisms critical to the performance of the polymer for applications.