Influence of very low doses of mediators on fungal laccase activity - nonlinearity beyond imagination.

Elzbieta Malarczyk, Janina Kochmanska-Rdest, Anna Jarosz-Wilkolazka
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引用次数: 17

Abstract

Laccase, an enzyme responsible for aerobic transformations of natural phenolics, in industrial applications requires the presence of low-molecular substances known as mediators, which accelerate oxidation processes. However, the use of mediators is limited by their toxicity and the high costs of exploitation. The activation of extracellular laccase in growing fungal culture with highly diluted mediators, ABTS and HBT is described. Two high laccase-producing fungal strains, Trametes versicolor and Cerrena unicolor, were used in this study as a source of enzyme. Selected dilutions of the mediators significantly increased the activity of extracellular laccase during 14 days of cultivation what was distinctly visible in PAGE technique and in colorimetric tests. The same mediator dilutions increased demethylation properties of laccase, which was demonstrated during incubation of enzyme with veratric acid. It was established that the activation effect was assigned to specific dilutions of mediators. Our dose-response dilution process smoothly passes into the range of action of homeopathic dilutions and is of interest for homeopaths.

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极低剂量介质对真菌漆酶活性的影响——超出想象的非线性。
漆酶是一种负责天然酚类有氧转化的酶,在工业应用中需要被称为介质的低分子物质的存在,这种介质可以加速氧化过程。然而,介质的使用受到其毒性和开发成本高的限制。本文描述了用高度稀释的培养基ABTS和HBT培养真菌时细胞外漆酶的激活情况。本研究以两种高产漆酶的真菌菌株,versicoltrametes和Cerrena unicolor作为酶的来源。在14天的培养过程中,选定的稀释培养基显著增加了细胞外漆酶的活性,这在PAGE技术和比色试验中是明显可见的。同样的介质稀释增加了漆酶的去甲基化特性,这在酶与戊二酸的孵育过程中得到了证明。结果表明,活化效应与特定稀释度的介质有关。我们的剂量反应稀释过程顺利进入顺势疗法稀释的作用范围,是顺势疗法的兴趣。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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