{"title":"Molecular characterization and expression analysis of Acmago and AcY14 in Antrodia cinnamomea","authors":"Fang-Hua Chu , Yu-Rong Chen , Chen-Hsien Lee , Tun-Tschu Chang","doi":"10.1016/j.mycres.2009.01.012","DOIUrl":null,"url":null,"abstract":"<div><p>Mago nashi (Mago) and Y14 proteins, highly conserved among eukaryotes, participate in mRNA localization and splicing, and as such play important roles in oogenesis, embryogenesis and germ-line sex determination during animal development. Here we identified <em>mago</em> (<em>Acmago</em>) and <em>Y14</em> (<em>AcY14</em>) homologues derived from <em>Antrodia cinnamomea. Acmago</em> encodes 149 amino acids and A<em>cY14</em> encodes 168 amino acids. Multiple amino acid sequence alignment as well as secondary and tertiary structure prediction showed that AcMago and AcY14 have similar protein structure to the reported crystal structures of other Mago and Y14 proteins. During fungal development both <em>Acmago</em> and <em>AcY14</em> genes were abundantly expressed in natural basidiomes. This is the first report of the molecular characterization and expression analysis of the <em>mago</em> and <em>Y14</em> genes from fungi.</p></div>","PeriodicalId":19045,"journal":{"name":"Mycological research","volume":"113 5","pages":"Pages 577-582"},"PeriodicalIF":0.0000,"publicationDate":"2009-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mycres.2009.01.012","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycological research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0953756209000240","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
Mago nashi (Mago) and Y14 proteins, highly conserved among eukaryotes, participate in mRNA localization and splicing, and as such play important roles in oogenesis, embryogenesis and germ-line sex determination during animal development. Here we identified mago (Acmago) and Y14 (AcY14) homologues derived from Antrodia cinnamomea. Acmago encodes 149 amino acids and AcY14 encodes 168 amino acids. Multiple amino acid sequence alignment as well as secondary and tertiary structure prediction showed that AcMago and AcY14 have similar protein structure to the reported crystal structures of other Mago and Y14 proteins. During fungal development both Acmago and AcY14 genes were abundantly expressed in natural basidiomes. This is the first report of the molecular characterization and expression analysis of the mago and Y14 genes from fungi.
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