Does pea lectin expressed transgenically in oilseed rape (Brassica napus) influence honey bee (Apis mellifera) larvae?

Environmental biosafety research Pub Date : 2007-10-01 Epub Date: 2007-11-28 DOI:10.1051/ebr:2007039
Anna Lehrman
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引用次数: 21

Abstract

The European honey bee (Apis mellifera) is important both for pollination and for honey production. Pollen is the major protein source for bees, which exposes them directly to changes in pollen quality e.g. through genetic engineering. In order to create a worst case scenario regarding pea lectin (PSL) expressed transgenically in oilseed rape anthers and pollen, the maximum amount of dried pollen that could be mixed in an artificial diet without negatively affecting larval performance (1.5% w/w) was fed to bee larvae. Pollen from two transgenic plant lines expressing PSL up to 1.2% of total soluble protein and pollen from one non-transgenic line was added to the same diet and used as a pollen control. When these three pollen diets and the control diet (without added pollen) were compared, no negative effect from the pollen of the transgenic plants could be detected on larval mortality, weight, or development time. An increased weight and a reduced developmental time were recorded for larvae on all diets containing pollen when compared to the diet without pollen.

油菜(Brassica napus)中转基因表达的豌豆凝集素会影响蜜蜂(Apis mellifera)幼虫吗?
欧洲蜜蜂(Apis mellifera)对授粉和蜂蜜生产都很重要。花粉是蜜蜂的主要蛋白质来源,这使它们直接面临花粉质量的变化,例如通过基因工程。为了对油菜花药和花粉中转基因表达的豌豆凝集素(PSL)做出最坏的设想,我们给蜜蜂幼虫喂食了最大剂量的干花粉,这种干花粉可以混合在人工饲料中,而不会对幼虫的表现产生负面影响(1.5% w/w)。在同一日粮中还添加了两个转基因植物品系的花粉,其表达的 PSL 可溶性蛋白总量最高可达 1.2%,同时还添加了一个非转基因品系的花粉作为花粉对照。比较这三种花粉日粮和对照日粮(未添加花粉),未发现转基因植物花粉对幼虫死亡率、体重或发育时间有负面影响。与不添加花粉的日粮相比,所有含有花粉的日粮都能增加幼虫的体重并缩短发育时间。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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