Facilitating co-existence by tracking gene dispersal in conventional potato systems with microsatellite markers.

Environmental biosafety research Pub Date : 2007-10-01 Epub Date: 2007-11-28 DOI:10.1051/ebr:2007033
Carloalberto Petti, Conor Meade, Martin Downes, Ewen Mullins
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引用次数: 11

Abstract

Based on international findings, Irish co-existence guidelines for the cultivation of GM potato stipulate that an isolation distance of 20 m is required to minimize the spread of transgenic pollen in accordance with required labeling thresholds. As potato tolerant to Phytophthora infestans is the most applicable GM crop from an Irish context, we tested the efficacy of this isolation distance under Irish environmental conditions using the conventional variety Désirée as a pollen donor and the male-sterile variety British Queen as a pollen receptor. Gene flow was determined by scoring for berry presence on receptor plants and confirmed using a microsatellite marker system designed to assess paternity in F(1) seedlings. 99.1% of seedlings recovered were identified as having Désirée paternity. Whereas 19.9% (140/708) of total berries formed on receptor plants occurred at a distance of 21 m from the pollen source, only 4 of these berries bore viable true potato seed (TPS), from which 23 TPS germinated. TPS-bearing berry formation was negatively correlated with distance from the pollen source, and although overall distribution of berries and seeds was non-random across the plot, no significant correlation was evident with respect to wind direction. Microsatellite markers were also used to confirm that the foraging beetle Meligethes aeneus is a vector for the transmission of potato pollen, but a more detailed statistical analysis of this dataset was limited by inclement weather during the trial. To conclude, we recommend that a two-tiered system be established in regard to establishing isolation distances for the experimental trial and commercial cultivation of GM potato in Ireland, and that responsible crop management be adopted to minimize the establishment of TPS-derived volunteers, which we have noted will emerge through a rotation as a result of pollen-mediated gene flow.

利用微卫星标记跟踪基因在传统马铃薯系统中的扩散,促进共存。
根据国际上的研究结果,爱尔兰转基因马铃薯种植共存指南规定,根据要求的标签阈值,需要20米的隔离距离,以最大限度地减少转基因花粉的传播。由于耐疫霉的马铃薯是爱尔兰环境下最适用的转基因作物,我们在爱尔兰环境条件下以常规品种dsisi作为花粉供体,以雄性不育品种英国女王作为花粉受体,测试了这种隔离距离的有效性。通过对受体植株上浆果的存在进行评分来确定基因流,并使用设计用于评估F(1)幼苗父系的微卫星标记系统进行确认。99.1%的恢复的幼苗被鉴定为具有dsamsipras父系。而在距离花粉源21 m处形成的果实占19.9%(140/708),其中只有4个果实具有活的马铃薯种子(TPS),其中23个种子萌发。果实形成与离花粉源的距离呈负相关,尽管果实和种子在整块地的总体分布是非随机的,但与风向的相关性不显著。微卫星标记也证实了觅食甲虫Meligethes aeneus是马铃薯花粉传播的媒介,但由于试验期间恶劣的天气,对该数据集进行更详细的统计分析受到限制。最后,我们建议建立一个双层系统,为爱尔兰转基因马铃薯的实验试验和商业种植建立隔离距离,并采取负责任的作物管理,以尽量减少tps衍生志愿者的建立,我们已经注意到,由于花粉介导的基因流动,这种志愿者将通过轮作出现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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