High resolution colonoscopy in live mice

IF 13.1 1区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Nature Protocols Pub Date : 2007-01-25 DOI:10.1038/nprot.2006.446

C Becker, M C Fantini, M F Neurath

引用次数: 301

Abstract

Endoscopy in humans is a powerful method for physicians to examine the gut for inflammatory or neoplastic changes. In medical and immunological research, animal models of intestinal diseases are established key tools to investigate the mucosal immune system, colitis and cancer development in the gut. Moreover, such models represent valid systems for testing of novel drugs. In the past, mice had to be killed in order to analyze colitis activity and tumor development. The following protocol describes a method to perform high resolution endoscopic monitoring of live mice. Mice developing colitis or colonic tumors are anesthetized and examined with a miniendoscope. The endoscope is introduced via the anus and the colon is carefully insufflated with an air pump. Endoscopic pictures obtained are of high quality and allow the monitoring and grading of tumors and inflammation. In addition, colonic biopsies can be taken. This protocol can be completed within 1 h.

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活体小鼠高分辨率结肠镜检查

人体内窥镜检查是医生检查肠道炎症或肿瘤变化的有效方法。在医学和免疫学研究中，肠道疾病动物模型是研究肠道粘膜免疫系统、结肠炎和癌症发展的重要工具。此外，此类模型还是测试新型药物的有效系统。过去，为了分析结肠炎的活动和肿瘤的发展，必须杀死小鼠。以下方案介绍了一种对活体小鼠进行高分辨率内窥镜监测的方法。对患上结肠炎或结肠肿瘤的小鼠进行麻醉，并用微型内窥镜进行检查。内窥镜从肛门进入，用气泵小心地对结肠进行充气。获得的内窥镜图像质量很高，可以对肿瘤和炎症进行监测和分级。此外，还可以进行结肠活检。该方案可在 1 小时内完成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Protocols 生物-生化研究方法

CiteScore

29.10

自引率

0.70%

发文量

128

审稿时长

4 months

期刊介绍： Nature Protocols focuses on publishing protocols used to address significant biological and biomedical science research questions, including methods grounded in physics and chemistry with practical applications to biological problems. The journal caters to a primary audience of research scientists and, as such, exclusively publishes protocols with research applications. Protocols primarily aimed at influencing patient management and treatment decisions are not featured. The specific techniques covered encompass a wide range, including but not limited to: Biochemistry, Cell biology, Cell culture, Chemical modification, Computational biology, Developmental biology, Epigenomics, Genetic analysis, Genetic modification, Genomics, Imaging, Immunology, Isolation, purification, and separation, Lipidomics, Metabolomics, Microbiology, Model organisms, Nanotechnology, Neuroscience, Nucleic-acid-based molecular biology, Pharmacology, Plant biology, Protein analysis, Proteomics, Spectroscopy, Structural biology, Synthetic chemistry, Tissue culture, Toxicology, and Virology.