Characterization of the rabbit HKα2 gene promoter

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2006-10-01 DOI:10.1016/j.bbaexp.2006.08.007

Deborah L. Zies , Michelle L. Gumz , Charles S. Wingo , Brian D. Cain

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引用次数: 3

Abstract

The HKα2 gene directs synthesis of the HKα2 subunit of the H⁺, K⁺-ATPase. In the kidney and colon, the gene is highly expressed and is thought to play a role in potassium (K⁺) conservation. The rabbit has been an important experimental system for physiological studies of ion transport in the kidney, so the rabbit HKα2 gene has been cloned and characterized. The genomic clones and the previously reported HKα2a and HKα2c subunit cDNAs provided a means to address several issues regarding the structure and expression of the HKα2 gene. First, the genomic organization established that the rabbit HKα2 gene was unambiguously homologous to the mouse HKα2 gene and the human ATP1AL1 gene. Second, the mapping of the transcription start site for the alternate transcript, HKα2c, confirmed that it was an authentic rabbit transcript. Finally, isolation of DNA from the 5′ end of the HKα2 gene enabled us to initiate studies on its regulation in the rabbit cortical collecting duct. The promoter and two putative negative regulatory regions were identified and the effect of cell confluency on gene expression was studied.

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兔HKα2基因启动子的鉴定

HKα2基因指导H+， K+- atp酶HKα2亚基的合成。在肾脏和结肠中，该基因高度表达，被认为在钾(K+)保护中起作用。兔是离子在肾脏转运生理研究的重要实验系统，因此兔HKα2基因已被克隆并表征。基因组克隆和先前报道的HKα2a和HKα2c亚基cdna提供了一种解决HKα2基因结构和表达的几个问题的方法。首先，基因组组织确定兔HKα2基因与小鼠HKα2基因和人类ATP1AL1基因具有明确的同源性。其次，另一个转录本HKα2c的转录起始位点的定位证实了它是一个真实的兔转录本。最后，我们从HKα2基因的5 '端分离DNA，开始了其在兔皮质集管中的调控研究。确定了启动子和两个可能的负调控区域，并研究了细胞融合对基因表达的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression

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