Simultaneous analysis of typical halogenated endocrine disrupting chemicals and metal(loid)s in human hair

IF 8 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Science of the Total Environment Pub Date : 2020-05-20 DOI:10.1016/j.scitotenv.2020.137300

Mengyuan Ren , Xiaoqian Jia , Jiazhang Shi , Lailai Yan , Zewu Li , Changxin Lan , Junxi Chen , Nan Li , Kexin Li , Jing Huang , Shaowei Wu , Qun Lu , Zhiwen Li , Bin Wang , Jianmeng Liu

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引用次数: 7

Abstract

Hair analysis has been an important approach in evaluating population exposure to various environmental factors. To meet the requirements of human environmental epidemiology studies, we aimed to develop an efficient method for simultaneous analysis of various metal(loid)s and some typical environmental halogenated endocrine disrupting chemicals (hEDCs) (i.e., polychlorinated biphenyls, polybrominated diphenyl ethers, and organochlorine pesticides, as well as some of their hydroxyl substituted metabolites) in a single hair sample. The hair was washed successively with surfactant solutions, methanol solvent, and deionized water to remove impurities attached to the hair surface. Efficiency was comprehensively compared among various washing strategies. The hair sample was further pulverized into fine powder with a median diameter (25th–75th percentile) of 8.6 (5.9–13.5) μm. The hair organic components were extracted by acetonitrile solvent and compared with the microwave-assisted extraction method. The hEDCs in the supernatant acetonitrile phase were quantified by gas chromatography–mass spectrometry, and the metal(loid)s in the precipitate hair were further analyzed by inductively coupled plasma mass spectrometry. Our developed method was further applied to analyze the hair samples of 165 pregnant women. The results showed that particles attached to the surface of the hair could not be washed off completely. However, we proposed a protocol framework to wash hair with relatively high efficience, which includes warm water incubation, and use of surfactant and organic solvent. The recoveries of the concerned hEDCs and metal(loid)s were overall in the range of 80% to 120%. For the women population, the method can efficiently recognize the typical exposure characteristics of the concerned hEDCs and metal(loid)s. Our study significantly ameliorated the deficiencies of the traditional hair washing strategy and developed an efficient method for simultaneous analysis of various metal(loid)s and hEDCs in a single hair sample. This method will provide important support for population complex exposure analysis and facilitate environmental exposome studies.

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人发中典型卤代内分泌干扰化学物质和金属(样物质)的同步分析

毛发分析已成为评估人群暴露于各种环境因素的重要方法。为了满足人类环境流行病学研究的需要，我们旨在建立一种同时分析单个头发样品中各种金属(样物质)和一些典型的环境卤代内分泌干扰物(hEDCs)(即多氯联苯、多溴联苯醚和有机氯农药，以及它们的一些羟基取代代谢物)的有效方法。用表面活性剂溶液、甲醇溶剂、去离子水依次清洗毛发，去除附着在毛发表面的杂质。综合比较了各种洗涤策略的效率。头发样品进一步粉碎成细粉，中位直径(25 - 75百分位)为8.6 (5.9-13.5)μm。用乙腈溶剂提取头发中的有机成分，并与微波辅助提取法进行比较。用气相色谱-质谱法测定上清乙腈相中的hedc，用电感耦合等离子体质谱法进一步测定沉淀毛中的金属(样蛋白)s。我们开发的方法进一步应用于分析165名孕妇的头发样本。结果表明，附着在头发表面的颗粒不能完全洗掉。然而，我们提出了一个相对高效的洗发方案框架，包括温水培养，表面活性剂和有机溶剂的使用。所涉及的hedc和金属(样)s的回收率总体在80% ~ 120%之间。对于女性人群，该方法可以有效识别出相关hedc和金属(类)物质的典型暴露特征。我们的研究显著改善了传统洗发策略的不足，并开发了一种有效的方法来同时分析单个头发样本中的各种金属(样物质)和hedc。该方法将为人群复杂暴露分析和环境暴露研究提供重要支持。

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来源期刊

Science of the Total Environment 环境科学-环境科学

CiteScore

17.60

自引率

10.20%

发文量

8726

审稿时长

2.4 months

期刊介绍： The Science of the Total Environment is an international journal dedicated to scientific research on the environment and its interaction with humanity. It covers a wide range of disciplines and seeks to publish innovative, hypothesis-driven, and impactful research that explores the entire environment, including the atmosphere, lithosphere, hydrosphere, biosphere, and anthroposphere. The journal's updated Aims & Scope emphasizes the importance of interdisciplinary environmental research with broad impact. Priority is given to studies that advance fundamental understanding and explore the interconnectedness of multiple environmental spheres. Field studies are preferred, while laboratory experiments must demonstrate significant methodological advancements or mechanistic insights with direct relevance to the environment.