A novel member of the NSF family in the corn earworm, Helicoverpa zea: molecular cloning, developmental expression, and tissue distribution

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression Pub Date : 2006-03-01 DOI:10.1016/j.bbaexp.2006.04.001

Wei-Hua Xu , Qi-Rui Zhang , David L. Denlinger

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引用次数: 2

Abstract

N-ethylmaleimide-sensitive fusion protein (NSF) is an ATPase that plays an essential role in intracellular membrane transport events. We report the cloning of a cDNA encoding NSF from the noctuid moth, Helicoverpa zea (Hez), a major agricultural pest. The amino acid sequence deduced from the cDNA indicates that Hez-NSF has 88%, 73%, and 70% identities to NSF from Manduca sexta, Aedes aegypti, and Drosophila melanogaster, respectively. Northern hybridization analysis clearly shows a 4.4 kb mRNA, corresponding in size to the cDNA present in the brain–suboesophageal ganglion (SG) complex of pupae. The NSF transcript is present in components of the central nervous system, including the brain, SG, thoracic ganglion and abdominal ganglion, but is not present in nonneural tissues. A developmental profile of gene expression revealed that Hez-NSF mRNA is present throughout the embryonic, larval, and pupal development.

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玉米穗虫NSF家族新成员:分子克隆、发育表达和组织分布

n -乙基马来酰亚胺敏感融合蛋白(NSF)是一种atp酶，在细胞膜内转运事件中起重要作用。本文报道了一种主要农业害虫——夜蛾(Helicoverpa zea, Hez)的NSF编码cDNA的克隆。cDNA序列分析表明，Hez-NSF与雄性曼都卡蚊(Manduca sexta)、埃及伊蚊(Aedes aegypti)和黑腹果蝇(Drosophila melanogaster)分别具有88%、73%和70%的同源性。Northern杂交分析清楚地显示一个4.4 kb的mRNA，其大小与蛹脑-食管下神经节(SG)复合体中的cDNA相对应。NSF转录本存在于中枢神经系统的组成部分，包括脑、SG、胸神经节和腹神经节，但不存在于非神经组织中。基因表达的发育谱显示，Hez-NSF mRNA存在于整个胚胎、幼虫和蛹的发育过程中。

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Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression

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