{"title":"Molecular characterization of a bHLH transcription factor involved in Arabidopsis abscisic acid-mediated response","authors":"Jiyoung Kim, Ho-Young Kim","doi":"10.1016/j.bbaexp.2006.03.002","DOIUrl":null,"url":null,"abstract":"<div><p>The suppression subtractive hybridization (SSH) method was used to isolate abscisic acid (ABA)-regulated genes in <em>Arabidopsis</em>. We report that the <em>AtAIG1</em> gene is up-regulated by ABA and the <em>AtAIG1</em> encodes a basic/helix–loop–helix (bHLH)-type transcription factor in <em>Arabidopsis</em>. The AtAIG1 was targeted into nucleus and bound to the E-box–DNA sequence, which is found in the promoter regions of many ABA-responsive genes. Functional analyses with the <em>ataig1</em> knockout mutants revealed that the mutant plants show enhanced sensitivity to ABA. In addition, the <em>ataig1</em> plants showed reduced expression of ABA-responsive genes, such as <em>RD29A</em> and <em>RD22</em>. Thus, these functional analyses suggest that the AtAIG1 protein plays an important role in ABA-mediated signal transduction pathway.</p></div>","PeriodicalId":100161,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","volume":"1759 3","pages":"Pages 191-194"},"PeriodicalIF":0.0000,"publicationDate":"2006-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbaexp.2006.03.002","citationCount":"30","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167478106000388","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 30
Abstract
The suppression subtractive hybridization (SSH) method was used to isolate abscisic acid (ABA)-regulated genes in Arabidopsis. We report that the AtAIG1 gene is up-regulated by ABA and the AtAIG1 encodes a basic/helix–loop–helix (bHLH)-type transcription factor in Arabidopsis. The AtAIG1 was targeted into nucleus and bound to the E-box–DNA sequence, which is found in the promoter regions of many ABA-responsive genes. Functional analyses with the ataig1 knockout mutants revealed that the mutant plants show enhanced sensitivity to ABA. In addition, the ataig1 plants showed reduced expression of ABA-responsive genes, such as RD29A and RD22. Thus, these functional analyses suggest that the AtAIG1 protein plays an important role in ABA-mediated signal transduction pathway.
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