A rapid and sensitive UHPLC-FT-ICR MS/MS method for identification of chemical constituents in Rhodiola crenulata extract, rat plasma and rat brain after oral administration

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2016-11-01 Epub Date: 2016-07-05 DOI:10.1016/j.talanta.2016.07.014

Fei Han, Yanting Li, Li Ma, Tianfeng Liu, Yawen Wu, Rui Xu, Aihua Song, Ran Yin

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引用次数: 37

Abstract

A rapid and sensitive UHPLC-FT-ICR MS/MS method was developed for the first time to analyze the extract of Rhodiola crenulata and the constituents absorbed into rat blood and brain after oral administration. Under the optimized conditions, a total of 64 chemical constituents were identified or tentatively characterized in vitro in 30 min, and also 24 and 9 chemical constituents were detected in rat plasma and brain respectively, by comparing the retention time, accurate mass and/or MS/MS data of blank and dosed sample. The results indicated that the developed UHPLC-FT-ICR MS/MS method was suitable for detection and identifying the chemical constituents in Rhodiola crenulata extract, rat plasma and rat brain, and it could be used as a powerful and reliable analytical strategy for rapid identification of chemical constituents in vitro and in vivo for other traditional Chinese herbal medicines (TCMs). Furthermore, the detected chemical constituents in rat brain could be speculated to be the pharmacodynamic substances of Rhodiola crenulata for Alzheimer's disease (AD) and it could also provide useful chemical information for further mass spectrometry imaging and bioactive substances research on Rhodiola crenulata.

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建立快速、灵敏的UHPLC-FT-ICR质谱联用方法，测定口服红景天提取物、大鼠血浆和大鼠脑中的化学成分

首次建立了快速、灵敏的UHPLC-FT-ICR MS/MS方法，对口服红景天提取物及其在大鼠血液和脑内的吸收成分进行分析。在优化条件下，通过比较空白和给药样品的保留时间、准确质量和/或MS/MS数据，在30 min内共鉴定或初步表征了64种化学成分，并在大鼠血浆和脑中分别检测到24种和9种化学成分。结果表明，所建立的UHPLC-FT-ICR MS/MS方法适用于红景天提取物、大鼠血浆和大鼠脑中化学成分的检测和鉴定，可作为其他中草药体外和体内化学成分快速鉴定的有力、可靠的分析策略。此外，在大鼠脑内检测到的化学成分可推测为红景天治疗阿尔茨海默病(AD)的药效学物质，也可为红景天进一步的质谱成像和生物活性物质研究提供有用的化学信息。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.