{"title":"[Protective effects of polarizing cardioplegia with Na(+) channel inhibitor in ex vivo rat heart preservation].","authors":"Song-tao Tan, Shuang-qiang Yang, Chao-kun Yang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the protective effects of polarizing cardioplegia with Na(+) channel inhibitor tetrodotoxin (TTX) in ex vivo rat heart preservation.</p><p><strong>Methods: </strong>Using a Langendorff preparation, Wistar rat hearts were arrested and preserved with St. Thomas (STH) solution (n=10) or with polarizing cardioplegia (TTX, n=10) for 7 h in hypothermic storage (10 degrees Celsius). All the hearts then underwent 30 min of reperfusion. Pre-ischemia and post-ischemia indexes of the rat hearts were determined, including the hemodynamic parameters, myocardial enzymology, ATP content and ultrastructural changes.</p><p><strong>Results: </strong>The recovery of hemodynamic parameters of the hearts in TTX group were better than those in STH group (P<0.01). In comparison with STH group, the leakage of creatine kinase (CK) and lactate dehydrogenase (LDH) in TTX group was significantly lower (P<0.05) and ATP level maintained a relative high level with better protected myocardial ultrastructure.</p><p><strong>Conclusion: </strong>TTX polarizing cardioplegia provides more effective long-term hypothermic preservation of isolated rat hearts than STH.</p>","PeriodicalId":11097,"journal":{"name":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To investigate the protective effects of polarizing cardioplegia with Na(+) channel inhibitor tetrodotoxin (TTX) in ex vivo rat heart preservation.
Methods: Using a Langendorff preparation, Wistar rat hearts were arrested and preserved with St. Thomas (STH) solution (n=10) or with polarizing cardioplegia (TTX, n=10) for 7 h in hypothermic storage (10 degrees Celsius). All the hearts then underwent 30 min of reperfusion. Pre-ischemia and post-ischemia indexes of the rat hearts were determined, including the hemodynamic parameters, myocardial enzymology, ATP content and ultrastructural changes.
Results: The recovery of hemodynamic parameters of the hearts in TTX group were better than those in STH group (P<0.01). In comparison with STH group, the leakage of creatine kinase (CK) and lactate dehydrogenase (LDH) in TTX group was significantly lower (P<0.05) and ATP level maintained a relative high level with better protected myocardial ultrastructure.
Conclusion: TTX polarizing cardioplegia provides more effective long-term hypothermic preservation of isolated rat hearts than STH.
目的:探讨Na(+)通道抑制剂河豚毒素(TTX)极化停搏对离体大鼠心脏的保护作用。方法:采用Langendorff制剂，取Wistar大鼠心脏，经St. Thomas (STH)溶液(n=10)或极化停搏液(TTX, n=10)低温保存7 h(10℃)。所有心脏进行30min再灌注。测定大鼠心脏缺血前、缺血后的血流动力学参数、心肌酶学、三磷酸腺苷(ATP)含量及超微结构变化。结果:TTX组心脏血流动力学参数恢复优于STH组(p)。结论:TTX极化心脏骤停对离体大鼠心脏的长期低温保存效果优于STH组。
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