The diversity in sensitivity of TRPA1 and TRPV1 of various animals to polyphenols.

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Sayuri Takahashi, Mako Kurogi, Osamu Saitoh
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引用次数: 2

Abstract

The perception of tastes is sensed by the receptors that stimulate sensory cells. We previously reported that TRPA1 and TRPV1 channels expressed in the oral cavity of mammals, are activated by the auto-oxidized product of epigallocatechin gallate (oxiEGCG), a major astringent catechin in green tea. Here, we investigated and compared the sensitivity of TRPA1 and TRPV1 from various animals to astringent polyphenols. We selected three polyphenols, oxiEGCG, tannic acid and myricetin. HEK293T cells expressing TRPA1 or TRPV1 from mammal, bird, reptile, amphibian, and fish, were analyzed for their activation by the Ca2+-imaging. We found the apparent diversity in the polyphenol-sensitivity among various animals. Mammalian TRPs showed relatively higher sensitivity to polyphenols, and especially, human TRPA1 and TRPV1 could be activated by all of three polyphenols at 20 μM. Reptile TRP channels, however, were insensitive to any polyphenols examined. Moreover, the polyphenol-sensitivity of zebrafish TRPA1 and TRPV1 was quite different from that of medaka TRP channels. Since many polyphenols are present in plants and the sensing of polyphenols using TRP channels in the oral cavity might cause astringent taste, the observed diversity of the polyphenol-sensitivity of TRP channels might be involved in the divergence in the food habit of various animals.

不同动物TRPA1和TRPV1对多酚敏感性的差异。
味觉是由刺激感觉细胞的受体感知的。我们之前报道过TRPA1和TRPV1通道在哺乳动物口腔中表达,被绿茶中的一种主要收敛儿茶素表没食子儿茶素没食子酸酯(oxiEGCG)的自动氧化产物激活。在此,我们研究并比较了不同动物TRPA1和TRPV1对收敛多酚的敏感性。我们选择了三种多酚,氧egcg,单宁酸和杨梅素。通过Ca2+成像分析了哺乳动物、鸟类、爬行动物、两栖动物和鱼类中表达TRPA1或TRPV1的HEK293T细胞的活化情况。我们发现不同动物对多酚的敏感性存在明显差异。哺乳动物TRPs对多酚具有较高的敏感性,其中人类TRPA1和TRPV1在20 μM下均可被3种多酚激活。然而,爬行动物的TRP通道对所检测的任何多酚都不敏感。此外,斑马鱼TRPA1和TRPV1的多酚敏感性与medaka TRP通道有很大不同。由于植物中存在许多多酚,而口腔中TRP通道对多酚的感知可能会引起涩味,因此观察到的TRP通道对多酚敏感性的差异可能与各种动物饮食习惯的差异有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomedical Research-tokyo
Biomedical Research-tokyo 医学-医学:研究与实验
CiteScore
2.40
自引率
0.00%
发文量
19
审稿时长
>12 weeks
期刊介绍: Biomedical Research is peer-reviewed International Research Journal . It was first launched in 1990 as a biannual English Journal and later became triannual. From 2008 it is published in Jan-Apr/ May-Aug/ Sep-Dec..
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