MOF@MnO2nanocomposites prepared usingin situmethod and recyclable cholesterol oxidase-inorganic hybrid nanoflowers for cholesterol determination.

IF 2.8 4区 材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY
Dawei Xu, Cong Li, Yuqiu Zi, Dafeng Jiang, Fei Qu, Xian-En Zhao
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引用次数: 7

Abstract

In this work, through thein situgrowth of MnO2nanosheets on the surface of terbium metal-organic frameworks (Tb-MOFs), MOF@MnO2nanocomposites are prepared and the fluorescence of Tb-MOFs is quenched significantly by MnO2. Additionally, the hybrid nanoflowers are self-assembled by cholesterol oxidase (ChOx) and copper phosphate (Cu3(PO4)2·3H2O). Then a new strategy for cholesterol determination is developed based on MOF@MnO2nanocomposites and hybrid nanoflowers. Cholesterol is oxidized under the catalysis of hybrid nanoflowers to yield H2O2, which further reduces MnO2nanosheets into Mn2+. Hence, the fluorescence recovery of Tb-MOFs is positively correlated to the concentration of cholesterol in the range of 10 to 360μM. The limit of detection (LOD) of cholesterol is 1.57μM. On the other hand, the hierarchical and confined structure of ChOx-inorganic hybrid nanoflowers greatly improve the stability of the enzyme. The activity of hybrid nanoflowers remains at a high level for one week when stored at room temperature. Moreover, the hybrid nanoflowers can be collected by centrifugation and reused. The activity of hybrid nanoflowers can continue at a high level for five cycles of determination. Therefore, it can be concluded that the hybrid nanoflowers are more stable and more economic than free enzymes, and they show a similar sensitivity and specificity to cholesterol compared with free ChOx. Finally, this strategy has been further validated for the determination of cholesterol in serum samples with satisfactory recoveries.

MOF@MnO2nanocomposites制备的可回收胆固醇氧化酶-无机杂交纳米花用于胆固醇测定。
本文通过在铽金属有机骨架(Tb-MOFs)表面生长MnO2纳米片,制备了MOF@MnO2nanocomposites, MnO2显著猝灭了Tb-MOFs的荧光。此外,混合纳米花由胆固醇氧化酶(ChOx)和磷酸铜(Cu3(PO4)2·3H2O)自组装。然后开发了一种基于MOF@MnO2nanocomposites和杂交纳米花的胆固醇测定新策略。在杂化纳米花的催化下,胆固醇被氧化生成H2O2, H2O2进一步将mno2纳米片还原成Mn2+。因此,在10 ~ 360μM范围内,tb - mof的荧光回收率与胆固醇浓度呈正相关。胆固醇的检出限为1.57μM。另一方面,chox -无机杂交纳米花的分层和受限结构大大提高了酶的稳定性。在室温条件下,杂交纳米花的活性可保持一周的高水平。此外,杂交纳米花可以通过离心收集和重复使用。杂交纳米花的活性在5个测定周期内均能保持较高水平。因此,可以得出结论,杂交纳米花比游离酶更稳定,更经济,并且与游离ChOx相比,它们对胆固醇具有相似的敏感性和特异性。最后,该策略已被进一步验证,用于血清样品中胆固醇的测定,回收率令人满意。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nanotechnology
Nanotechnology 工程技术-材料科学:综合
CiteScore
7.10
自引率
5.70%
发文量
820
审稿时长
2.5 months
期刊介绍: The journal aims to publish papers at the forefront of nanoscale science and technology and especially those of an interdisciplinary nature. Here, nanotechnology is taken to include the ability to individually address, control, and modify structures, materials and devices with nanometre precision, and the synthesis of such structures into systems of micro- and macroscopic dimensions such as MEMS based devices. It encompasses the understanding of the fundamental physics, chemistry, biology and technology of nanometre-scale objects and how such objects can be used in the areas of computation, sensors, nanostructured materials and nano-biotechnology.
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