Repair of programmed DNA lesions in antibody class switch recombination: common and unique features.

Genome instability & disease Pub Date : 2021-01-01 Epub Date: 2021-03-26 DOI:10.1007/s42764-021-00035-0
Yafang Shang, Fei-Long Meng
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引用次数: 3

Abstract

The adaptive immune system can diversify the antigen receptors to eliminate various pathogens through programmed DNA lesions at antigen receptor genes. In immune diversification, general DNA repair machineries are applied to transform the programmed DNA lesions into gene mutation or recombination events with common and unique features. Here we focus on antibody class switch recombination (CSR), and review the initiation of base damages, the conversion of damaged base to DNA double-strand break, and the ligation of broken ends. With an emphasis on the unique features in CSR, we discuss recent advances in the understanding of DNA repair/replication coordination, and ERCC6L2-mediated deletional recombination. We further elaborate the application of CSR in end-joining, resection and translesion synthesis assays. In the time of the COVID-19 pandemic, we hope it help to understand the generation of therapeutic antibodies.

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抗体类开关重组中程序性DNA损伤的修复:共性与独特性。
适应性免疫系统通过抗原受体基因的程序化DNA损伤,使抗原受体多样化,从而消灭各种病原体。在免疫多样化中,一般的DNA修复机制被用来将程序化的DNA损伤转化为具有共同和独特特征的基因突变或重组事件。本文重点介绍了抗体类开关重组(CSR),并综述了碱基损伤的起始、受损碱基向DNA双链断裂的转化以及断裂端的连接。在强调CSR的独特特征的同时,我们讨论了DNA修复/复制协调和ercc6l2介导的缺失重组的最新进展。我们进一步阐述了CSR在末端连接、切除和平移合成分析中的应用。在COVID-19大流行期间,我们希望它有助于了解治疗性抗体的产生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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