An in situ-forming polyzwitterion hydrogel: Towards vitreous substitute application

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials Pub Date : 2021-10-01 DOI:10.1016/j.bioactmat.2021.02.029

Binbin He , Jianhai Yang , Yang Liu , Xianhua Xie , Huijie Hao , Xiaoli Xing , Wenguang Liu

{"title":"An in situ-forming polyzwitterion hydrogel: Towards vitreous substitute application","authors":"Binbin He , Jianhai Yang , Yang Liu , Xianhua Xie , Huijie Hao , Xiaoli Xing , Wenguang Liu","doi":"10.1016/j.bioactmat.2021.02.029","DOIUrl":null,"url":null,"abstract":"<div><p>Development of a biostable and biosafe vitreous substitute is highly desirable, but remains a grand challenge. Herein, we propose a novel strategy for constructing a readily administered vitreous substitute based on a thiol-acrylate clickable polyzwitterion macromonomer. A biocompatible multivinyl polycarboxybetaine (PCB-OAA) macromonomer is designed and synthesized, and mixed with dithiothreitol (DTT) via a Michael addition reaction to form a hydrogel in vitreous cavity. This resultant PCB-OAA hydrogel exhibits controllable gelation time, super anti-fouling ability against proteins and cells, excellent biocompatibility, and approximate key parameters to human vitreous body including equilibrium water content, density, optical properties, modulus. Remarkably, outperforming clinically used silicone oil in biocompatibility, this rapidly formed hydrogel in the vitreous cavity of rabbit eyes remains stable in vitreous cavity, showing an appealing ability to prevent significantly inflammatory response, fibrosis and complications such as raised intraocular pressure (IOP), and cataract formation. This zwitterionic polymer hydrogel holds great potential as a vitreous substitute.</p></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"6 10","pages":"Pages 3085-3096"},"PeriodicalIF":18.0000,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bioactmat.2021.02.029","citationCount":"14","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioactive Materials","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452199X21000888","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}

引用次数: 14

Abstract

Development of a biostable and biosafe vitreous substitute is highly desirable, but remains a grand challenge. Herein, we propose a novel strategy for constructing a readily administered vitreous substitute based on a thiol-acrylate clickable polyzwitterion macromonomer. A biocompatible multivinyl polycarboxybetaine (PCB-OAA) macromonomer is designed and synthesized, and mixed with dithiothreitol (DTT) via a Michael addition reaction to form a hydrogel in vitreous cavity. This resultant PCB-OAA hydrogel exhibits controllable gelation time, super anti-fouling ability against proteins and cells, excellent biocompatibility, and approximate key parameters to human vitreous body including equilibrium water content, density, optical properties, modulus. Remarkably, outperforming clinically used silicone oil in biocompatibility, this rapidly formed hydrogel in the vitreous cavity of rabbit eyes remains stable in vitreous cavity, showing an appealing ability to prevent significantly inflammatory response, fibrosis and complications such as raised intraocular pressure (IOP), and cataract formation. This zwitterionic polymer hydrogel holds great potential as a vitreous substitute.

Abstract Image

查看原文本刊更多论文

一种原位形成的多两性离子水凝胶:面向玻璃体的替代应用

开发一种生物稳定和生物安全的玻璃体替代品是非常可取的，但仍然是一个巨大的挑战。在此，我们提出了一种新的策略，以巯基丙烯酸酯可点击多两性离子大单体为基础，构建一种易于施用的玻璃体替代品。设计合成了一种生物相容性多乙烯基聚羧基甜菜碱(PCB-OAA)大单体，并与二硫苏糖醇(DTT)通过Michael加成反应在玻璃体腔内形成水凝胶。所得的多氯联苯-丙烯酸水凝胶具有可控制的凝胶时间，对蛋白质和细胞具有超强的抗污染能力，良好的生物相容性，并具有与人体玻璃体近似的关键参数，包括平衡含水量，密度，光学性质，模量。值得注意的是，在生物相容性方面优于临床使用的硅油，这种在兔眼玻璃体腔中快速形成的水凝胶在玻璃体腔中保持稳定，显示出明显的炎症反应、纤维化和并发症，如眼压升高(IOP)和白内障的形成。这种两性离子聚合物水凝胶作为玻璃体替代品具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioactive Materials Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

28.00

自引率

6.30%

发文量

436

审稿时长

20 days

期刊介绍： Bioactive Materials is a peer-reviewed research publication that focuses on advancements in bioactive materials. The journal accepts research papers, reviews, and rapid communications in the field of next-generation biomaterials that interact with cells, tissues, and organs in various living organisms. The primary goal of Bioactive Materials is to promote the science and engineering of biomaterials that exhibit adaptiveness to the biological environment. These materials are specifically designed to stimulate or direct appropriate cell and tissue responses or regulate interactions with microorganisms. The journal covers a wide range of bioactive materials, including those that are engineered or designed in terms of their physical form (e.g. particulate, fiber), topology (e.g. porosity, surface roughness), or dimensions (ranging from macro to nano-scales). Contributions are sought from the following categories of bioactive materials: Bioactive metals and alloys Bioactive inorganics: ceramics, glasses, and carbon-based materials Bioactive polymers and gels Bioactive materials derived from natural sources Bioactive composites These materials find applications in human and veterinary medicine, such as implants, tissue engineering scaffolds, cell/drug/gene carriers, as well as imaging and sensing devices.