Detection of 2-hydroxy-fatty acids and 2-hydroxy-fatty acid-containing ceramides in a gliding marine bacterium Aureispira marina.

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Kazuyoshi Kawahara, Hirokazu Iida, Akira Yokota
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引用次数: 1

Abstract

The cellular fatty acid composition of Aureispira marina IAM 15389T (JCM 23197T), a gliding bacterium isolated from the coastline of Thailand, was re-examined by using a standard MIDI method based on alkaline hydrolysis, and two other methods. The direct transesterification using 5% HCl/methanol or 4 M HCl hydrolysis followed by methyl esterification revealed that 2-hydroxy-15-methyl-hexadecanoic acid (2-OH-iso-C17:0) and 2-hydroxy-15-methyl-hexadecenoic acid (2-OH-iso-C17:1), which were not reported in a previous paper, were found to be major cellular fatty acids of this bacterium, and the amount of 2-OH-iso-C17:1 was even higher than that of arachidonic acid (C20:4), a characteristic polyunsaturated fatty acid present in this bacterium. These 2-hydroxy-fatty acids were contained in two cellular lipids that were relatively stable against alkaline hydrolysis. One of them was analyzed by mass spectrometry, 1H-nuclear magnetic resonance, and other chemical methods, and identified as a ceramide composed of 2-hydroxy-fatty acid and sphingosine of 19 carbons with three double bonds. A minor ceramide containing 18 carbon sphingosine with three double bonds was also detected.

2-羟基脂肪酸及含2-羟基脂肪酸的神经酰胺的检测。
采用一种基于碱性水解的标准MIDI法和另外两种方法,对分离自泰国海岸线的滑翔细菌Aureispira marina IAM 15389T (JCM 23197T)的细胞脂肪酸组成进行了重新检测。采用5% HCl/甲醇直接酯交换反应或4 M HCl水解后进行甲基酯化反应,发现2-羟基-15-甲基-十六烯酸(2-OH-iso-C17:0)和2-羟基-15-甲基-十六烯酸(2-OH-iso-C17:1)是该细菌的主要细胞脂肪酸,且2-OH-iso-C17:1的含量甚至高于花生四烯酸(C20:4)。多不饱和脂肪酸这种细菌中特有的多不饱和脂肪酸这些2-羟基脂肪酸包含在两种抗碱性水解相对稳定的细胞脂质中。其中一种经质谱、1h核磁共振等化学方法分析,鉴定为由2-羟基脂肪酸和鞘氨醇组成的神经酰胺,共19个碳,有3个双键。还检测到一种含有18碳鞘氨醇和3个双键的小神经酰胺。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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