Long non-coding RNA PITPNA-AS1 regulates UNC5B expression in papillary thyroid cancer via sponging miR-129-5p.

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Journal of Biological Markers Pub Date : 2021-03-01 Epub Date: 2021-03-11 DOI:10.1177/1724600820985528

Shijuan Mei, Huafeng Zong, Haicheng Zhou

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引用次数: 3

Abstract

Background: Long non-coding RNA (lncRNA) PITPNA antisense RNA 1 (PITPNA-AS1) expression characteristics, function, and mechanism in papillary thyroid cancer are unclear.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was applied for detecting PITPNA-AS1, UNC-5 netrin receptor B (UNC5B) mRNA, and miR-129-5p expressions in papillary thyroid cancer tissues and cell lines. EdU assay, cell counting kit-8 (CCK-8) assay, wound healing assay, and flow cytometry analysis were performed to investigate the biological functions of PITPNA-AS1 in papillary thyroid cancer. Dual-luciferase reporter assay was utilized for determining whether PITPNA-AS1 and miR-129-5p, as well as UNC5B and miR-129-5p could directly bind to each other. Western blot assay was employed for measuring UNC5B protein expression level in papillary thyroid cancer cell lines.

Results: PITPNA-AS1 and UNC5B expressions were markedly increased in papillary thyroid cancer tissues and cell lines while miR-129-5p expression was down-regulated. Knockdown of PITPNA-AS1 could significantly inhibit papillary thyroid cancer cell growth and migration and promote cell apoptosis while UNC5B overexpression plasmids or miR-129-5p inhibitors counteracted the knockdown effect of PITPNA-AS1 on papillary thyroid cancer cells. PITPNA-AS1 targeted miR-129-5p to repress its expression and miR-129-5p targeted UNC5B to repress its expression. Silencing PITPNA-AS1 reduced the expression of UNC5B via regulating miR-129-5p expression.

Conclusions: PITPNA-AS1 facilitated papillary thyroid cancer cell proliferation and migration, and suppressed apoptosis through miR-129-5p/UNC5B axis.

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长链非编码RNA PITPNA-AS1通过海绵miR-129-5p调控乳头状甲状腺癌中UNC5B的表达。

背景:长链非编码RNA (lncRNA)、PITPNA反义RNA 1 (PITPNA- as1)在甲状腺乳头状癌中的表达特征、功能及机制尚不清楚。方法:采用实时定量聚合酶链反应(qRT-PCR)检测甲状腺乳头状癌组织和细胞系中PITPNA-AS1、UNC-5网状蛋白受体B (UNC5B) mRNA和miR-129-5p的表达。采用EdU法、细胞计数试剂盒-8 (CCK-8)法、创面愈合法和流式细胞术分析，探讨PITPNA-AS1在甲状腺乳头状癌中的生物学功能。采用双荧光素酶报告基因法测定PITPNA-AS1与miR-129-5p、UNC5B与miR-129-5p能否直接结合。Western blot法检测UNC5B蛋白在甲状腺乳头状癌细胞中的表达水平。结果:甲状腺乳头状癌组织和细胞系中PITPNA-AS1、UNC5B表达明显升高，miR-129-5p表达下调。敲低PITPNA-AS1可显著抑制甲状腺乳头状癌细胞的生长和迁移，促进细胞凋亡，而UNC5B过表达质粒或miR-129-5p抑制剂可抵消PITPNA-AS1对甲状腺乳头状癌细胞的敲低作用。PITPNA-AS1靶向miR-129-5p抑制其表达，miR-129-5p靶向UNC5B抑制其表达。沉默PITPNA-AS1可通过调节miR-129-5p的表达来降低UNC5B的表达。结论:PITPNA-AS1通过miR-129-5p/UNC5B轴促进甲状腺乳头状癌细胞增殖和迁移，抑制细胞凋亡。

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来源期刊

International Journal of Biological Markers 医学-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

期刊介绍： IJBM is an international, online only, peer-reviewed Journal, which publishes original research and critical reviews primarily focused on cancer biomarkers. IJBM targets advanced topics regarding the application of biomarkers in oncology and is dedicated to solid tumors in adult subjects. The clinical scenarios of interests are screening and early diagnosis of cancer, prognostic assessment, prediction of the response to and monitoring of treatment.