Motor, sensitive, and vegetative recovery in rats with compressive spinal-cord injury after combined treatment with erythropoietin and whole-body vibration.

IF 1.9 4区医学 Q4 NEUROSCIENCES

Restorative neurology and neuroscience Pub Date : 2021-01-01 DOI:10.3233/RNN-201120

Svenja Rink, Maria Eleni Manthou, Julia Arnold, Merle Grigo, Paulina Dicken, Diana Saad Yousif Abdulla, Habib Bendella, Klaus Nohroudi, Doychin N Angelov

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引用次数: 2

Abstract

Background: Physical therapy with whole body vibration (WBV) following compressive spinal cord injury (SCI) in rats restores density of perisomatic synapses, improves body weight support and leads to a better bladder function. The purpose of the study was to determine whether the combined treatment with WBV plus erythropoietin (EPO) would further improve motor, sensory and vegetative functions after SCI in rats.

Methods: Severe compressive SCI at low thoracic level was followed by a single i.p. injection of 2,5μg (250 IU) human recombinant EPO. Physical therapy with WBV started on 14th day after injury and continued over a 12-week post injury period. Locomotor recovery, sensitivity tests and urinary bladder scores were analysed at 1, 3, 6, 9, and 12 weeks after SCI. The closing morphological measurements included lesion volume and numbers of axons in the preserved perilesional neural tissue bridges (PNTB).

Results: Assessment of motor performance sensitivity and bladder function revealed no significant effects of EPO when compared to the control treatments. EPO treatment neither reduced the lesion volume, nor increased the number of axons in PNTB.

Conclusions: The combination of WBV + EPO exerts no positive effects on hind limbs motor performance and bladder function after compressive SCI in rats.

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促红细胞生成素和全身振动联合治疗后脊髓压缩性损伤大鼠的运动、敏感和营养恢复。

背景:大鼠压缩性脊髓损伤(SCI)后全身振动物理治疗(WBV)可恢复核周围突触密度，改善体重支撑，改善膀胱功能。本研究的目的是确定WBV联合促红细胞生成素(EPO)是否能进一步改善大鼠脊髓损伤后的运动、感觉和营养功能。方法:重度低胸段压缩性脊髓损伤患者单次腹腔注射2.5 μg (250 IU)人重组EPO。WBV物理治疗于伤后第14天开始，并持续12周。在脊髓损伤后1、3、6、9和12周分析运动恢复、敏感性测试和膀胱评分。闭合形态学测量包括病变体积和保存的病灶周围神经组织桥(PNTB)的轴突数量。结果:运动表现敏感性和膀胱功能的评估显示，与对照组相比，EPO没有显著影响。EPO治疗既没有减少PNTB的病变体积，也没有增加轴突的数量。结论:WBV + EPO联合应用对大鼠压缩性脊髓损伤后后肢运动功能和膀胱功能无积极影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Restorative neurology and neuroscience 医学-神经科学

CiteScore

5.40

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： This interdisciplinary journal publishes papers relating to the plasticity and response of the nervous system to accidental or experimental injuries and their interventions, transplantation, neurodegenerative disorders and experimental strategies to improve regeneration or functional recovery and rehabilitation. Experimental and clinical research papers adopting fresh conceptual approaches are encouraged. The overriding criteria for publication are novelty, significant experimental or clinical relevance and interest to a multidisciplinary audience. Experiments on un-anesthetized animals should conform with the standards for the use of laboratory animals as established by the Institute of Laboratory Animal Resources, US National Academy of Sciences. Experiments in which paralytic agents are used must be justified. Patient identity should be concealed. All manuscripts are sent out for blind peer review to editorial board members or outside reviewers. Restorative Neurology and Neuroscience is a member of Neuroscience Peer Review Consortium.