Visualization of subdiffusive sites in a live single cell.

Journal of biological methods Pub Date : 2021-01-30 eCollection Date: 2021-01-01 DOI:10.14440/jbm.2021.348
Zeno Földes-Papp, Gerd Baumann, Long-Cheng Li
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Abstract

We measured anomalous diffusion in human prostate cancer cells which were transfected with the Alexa633 fluorescent RNA probe and co-transfected with enhanced green fluorescent protein-labeled argonaute2 protein by laser scanning microscopy. The image analysis arose from diffusion based on a "two-level system". A trap was an interaction site where the diffusive motion was slowed down. Anomalous subdiffusive spreading occurred at cellular traps. The cellular traps were not immobile. We showed how the novel analysis method of imaging data resulted in new information about the number of traps in the crowded and heterogeneous environment of a single human prostate cancer cell. The imaging data were consistent with and explained by our modern ideas of anomalous diffusion of mixed origins in live cells. Our original research presented in this study is significant as we obtained a complex diffusion mechanism in live single cells.

Abstract Image

Abstract Image

Abstract Image

活单细胞亚扩散部位的可视化。
用激光扫描显微镜观察了转染Alexa633荧光RNA探针和共转染增强型绿色荧光蛋白标记argonaute2蛋白的人前列腺癌细胞的异常扩散。图像分析产生于基于“两级系统”的扩散。陷阱是一个相互作用的地方,扩散运动被减缓。异常的亚弥漫性扩散发生在细胞陷阱。细胞陷阱不是固定的。我们展示了成像数据的新分析方法如何产生关于单个人类前列腺癌细胞拥挤和异质环境中陷阱数量的新信息。成像数据与我们现代关于活细胞中混合起源异常扩散的观点一致并得到了解释。我们在这项研究中提出的原始研究具有重要意义,因为我们获得了活的单细胞中复杂的扩散机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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