Intracellular host cell membrane remodelling induced by SARS-CoV-2 infection in vitro.

IF 2.4 4区生物学 Q4 CELL BIOLOGY

Biology of the Cell Pub Date : 2021-06-01 Epub Date: 2021-03-03 DOI:10.1111/boc.202000146

Lucio Ayres Caldas, Fabiana Avila Carneiro, Fabio Luis Monteiro, Ingrid Augusto, Luiza Mendonça Higa, Kildare Miranda, Amilcar Tanuri, Wanderley de Souza

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引用次数: 15

Abstract

Background information: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection induces an alteration in the endomembrane system of the mammalian cells. In this study, we used transmission electron microscopy and electron tomography to investigate the main structural alterations in the cytoplasm of Vero cells infected with a SARS-CoV-2 isolate from São Paulo state (Brazil).

Results: Different membranous structures derived from the zippered endoplasmic reticulum were observed along with virus assembly through membrane budding. Also, we demonstrated the occurrence of annulate lamellae in the cytoplasm of infected cells and the presence of virus particles in the perinuclear space.

Conclusions and significance: This study contributes to a better understanding of the cell biology of SARS-CoV-2 and the mechanisms of the interaction of the virus with the host cell that promote morphological changes, recruitment of organelles and cell components, in a context of a virus-induced membrane remodelling.

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SARS-CoV-2感染诱导的体外细胞内宿主细胞膜重塑。

背景资料:严重急性呼吸综合征冠状病毒-2 (SARS-CoV-2)感染诱导哺乳动物细胞内膜系统发生改变。在这项研究中，我们使用透射电子显微镜和电子断层扫描研究了来自巴西圣保罗州的SARS-CoV-2分离物感染Vero细胞细胞质的主要结构变化。结果:随着病毒通过膜出芽组装，在拉链内质网中观察到不同的膜结构。此外，我们还证实了在感染细胞的细胞质中出现环状片层，并且在核周空间中存在病毒颗粒。结论与意义:本研究有助于更好地理解SARS-CoV-2的细胞生物学，以及在病毒诱导的膜重构背景下，病毒与宿主细胞相互作用促进形态变化、细胞器和细胞成分募集的机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biology of the Cell 生物-细胞生物学

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal publishes original research articles and reviews on all aspects of cellular, molecular and structural biology, developmental biology, cell physiology and evolution. It will publish articles or reviews contributing to the understanding of the elementary biochemical and biophysical principles of live matter organization from the molecular, cellular and tissues scales and organisms. This includes contributions directed towards understanding biochemical and biophysical mechanisms, structure-function relationships with respect to basic cell and tissue functions, development, development/evolution relationship, morphogenesis, stem cell biology, cell biology of disease, plant cell biology, as well as contributions directed toward understanding integrated processes at the organelles, cell and tissue levels. Contributions using approaches such as high resolution imaging, live imaging, quantitative cell biology and integrated biology; as well as those using innovative genetic and epigenetic technologies, ex-vivo tissue engineering, cellular, tissue and integrated functional analysis, and quantitative biology and modeling to demonstrate original biological principles are encouraged.