A Selectable Biomarker in Hair Follicle Cycles - Cathepsins: A Preliminary Study in Murine.

IF 2.8 4区医学 Q2 DERMATOLOGY

Skin Pharmacology and Physiology Pub Date : 2021-01-01 Epub Date: 2021-02-15 DOI:10.1159/000509943

Jingzhu Bai, Zijian Gong, Qingfang Xu, Haiyan Chen, Qiaoping Chen, Ruihua Fang, Yue Zheng, Wei Lai

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引用次数: 1

Abstract

Background/objective: Hair cycle is regulated by many biological factors. Cathepsins are involved in various physiological processes in human skin. Here, we investigated the cathepsin expression and distribution changes in follicular growth cycles for better understanding the hair cycles and to explore new intervention measures.

Methods: The 24 mice (C57BL/6, female, 7-week old) were selected and removed the back hair via rosin/paraffin method. At Day 8, Day 20, and Day 25, biopsy on post-plucking area was done. Immunohistochemical staining, Western blot, and Q-PCR were used to test the cathepsin B/D/L/E.

Results: In anagen, cathepsins (B, D, L, and E) were distributed in the hair follicle matrix, inner hair root sheath, and hair. In catagen, cathepsins were mainly observed in un-apoptosis inner root sheath and outer root sheath. Expression of cathepsins B-mRNA and L-mRNA was decreased from anagen and catagen to telogen. Cathepsin D-mRNA was increased in catagen and then decreased in telogen. Cathepsin E-mRNA was decreased in catagen and slightly increased in telogen.

Conclusions: The distribution and expression of cathepsins B, D, L, and E in hair follicle changed with hair growth process which indicated that cathepsins might act as selectable biomarkers of hair cycle in different stages.

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毛囊周期中可选择的生物标志物组织蛋白酶:小鼠的初步研究。

背景/目的:头发周期受多种生物因素的调控。组织蛋白酶参与人体皮肤的各种生理过程。为了更好地了解毛囊生长周期，探索新的干预措施，我们研究了组织蛋白酶在毛囊生长周期中的表达和分布变化。方法:选取24只小鼠(C57BL/6，雌性，7周龄)，采用松香/石蜡法去除背毛。在第8天、第20天和第25天，对拔毛后区域进行活检。采用免疫组化染色、Western blot、Q-PCR检测组织蛋白酶B/D/L/E。结果:在毛发生长初期，组织蛋白酶(B、D、L、E)分布于毛囊基质、毛根内鞘和毛发中。在脱落原中，组织蛋白酶主要存在于未凋亡的根内鞘和根外鞘中。组织蛋白酶B-mRNA和L-mRNA的表达从生长、衰亡期到休止期均呈下降趋势。组织蛋白酶D-mRNA在休止期升高，休止期降低。组织蛋白酶E-mRNA在休止期略有升高，在休止期略有下降。结论:组织蛋白酶B、D、L和E在毛囊内的分布和表达随头发生长过程的变化而变化，表明组织蛋白酶可能是不同阶段头发周期的选择性生物标志物。

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来源期刊

Skin Pharmacology and Physiology 医学-皮肤病学

CiteScore

5.20

自引率

7.40%

发文量

审稿时长

>12 weeks

期刊介绍： In the past decade research into skin pharmacology has rapidly developed with new and promising drugs and therapeutic concepts being introduced regularly. Recently, the use of nanoparticles for drug delivery in dermatology and cosmetology has become a topic of intensive research, yielding remarkable and in part surprising results. Another topic of current research is the use of tissue tolerable plasma in wound treatment. Stimulating not only wound healing processes but also the penetration of topically applied substances into the skin, this novel technique is expected to deliver very interesting results.