Reproductive Toxicity of Theobroma cacao: Increase in Survival Index, Nongenotoxic, and Proimplantation Potential.

IF 3.4 Q2 TOXICOLOGY

Journal of Toxicology Pub Date : 2021-01-12 eCollection Date: 2021-01-01 DOI:10.1155/2021/6114672

I J Asiedu-Gyekye, T G Borovskaya, M E Poluektova, А V Vychuzhanina, Y А Shchemerovа, S I Kamalova, V A Grgoreva, P Amoateng, K E Kukuia, A A Kwapong, L Allotey Babington, S K Amponsah, B B N'guessan

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引用次数: 3

Abstract

Unsweetened natural cocoa (UNCP) was evaluated for reproductive toxicity in rats. A preliminary genotoxic potential was evaluated by the DNA comet assay test using C57Bl/6 mice. Both therapeutic dose (TD; 900 mg/kg) and high dose (HD; 9000 mg/kg) of UNCP were used. White Wistar rats were used in two experimental groups. The females received UNCP 15 days before crossing with untreated males. The males received UNCP for 48 days before mating with untreated females. Subacute toxicity was observed during a 14-day oral administration of UNCP. Results show that a high tail DNA% was observed with methyl mesylate administration in all tissues analysed. The lowest tail DNA% value was observed in the liver (1.64 ± 0.26) and kidney (1.63 ± 0.30) during UNCP (TD) administration. UNCP did not induce observable physical congenital malformations on the pubs of treated female and male rats, lacks genotoxic potential, and did not adversely affect pregnancy index, pub weights, and survival index, but UNCP exhibited proimplantation potential (p > 0.05).

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可可的生殖毒性:增加存活指数、非基因毒性和着床前潜能。

研究了无糖天然可可(UNCP)对大鼠生殖毒性的影响。以C57Bl/6小鼠为实验对象，通过DNA彗星试验初步评价了其基因毒性。治疗剂量(TD;900 mg/kg)和高剂量(HD;UNCP用量为9000 mg/kg。选用Wistar大鼠作为两个实验组。雌性在与未经处理的雄性交配前15天接受UNCP治疗。雄鼠在与未处理的雌鼠交配前48天接受UNCP治疗。口服UNCP 14天观察到亚急性毒性。结果表明，甲磺酸甲酯在所有组织中观察到较高的尾部DNA%。尾DNA%值最低的部位为肝脏(1.64±0.26)和肾脏(1.63±0.30)。UNCP对雌性和雄性大鼠均无明显的生理先天畸形，无遗传毒性，对妊娠指数、体重和生存指数均无不良影响，但有一定的着床前潜能(p > 0.05)。

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来源期刊

Journal of Toxicology TOXICOLOGY-

CiteScore

5.50

自引率

3.40%

发文量

审稿时长

10 weeks

期刊介绍： Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.