[Method of polymerase chain reaction in toxoplasmosis diagnosis].

Zuzana Cermáková, Lenka Plísková, Petr Prásil, Olga Rysková
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Abstract

Since introduction of polymerase chain reaction for the detection of DNA Toxoplasma gondii 398 biological samples from 301 patients were examined from 2000 to 2003. Positive finding of toxoplasmosis DNA we noted in 23 cases. Polymerase chain reaction enables exact and fast diagnosis of the actual parasitemia Toxoplasma gondii, which is important especially in the pregnant patients, new-born with suspicion on congenital toxoplasmosis and in the patients with immunosupression.

聚合酶链反应在弓形虫病诊断中的应用
自2000年至2003年采用聚合酶链反应检测刚地弓形虫DNA以来,共对301例患者的398份生物样本进行了检测。23例检测弓形虫病DNA阳性。聚合酶链反应能够准确、快速地诊断弓形虫病,尤其对孕妇、怀疑先天性弓形虫病的新生儿和免疫抑制患者具有重要意义。
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