Maria Lucia Taylor , Guillermo M. Ruíz-Palacios , María del Rocío Reyes-Montes , Gabriela Rodríguez-Arellanes , Laura E. Carreto-Binaghi , Esperanza Duarte-Escalante , Aurora Hernández-Ramírez , Armando Pérez , Roberto O. Suárez-Alvarez , Yuri A. Roldán-Aragón , Rafael Romero-Martínez , Jorge H. Sahaza-Cardona , José Sifuentes-Osornio , Luis E. Soto-Ramírez , Gabriela R. Peña-Sandoval
{"title":"Identification of the infectious source of an unusual outbreak of histoplasmosis, in a hotel in Acapulco, state of Guerrero, Mexico","authors":"Maria Lucia Taylor , Guillermo M. Ruíz-Palacios , María del Rocío Reyes-Montes , Gabriela Rodríguez-Arellanes , Laura E. Carreto-Binaghi , Esperanza Duarte-Escalante , Aurora Hernández-Ramírez , Armando Pérez , Roberto O. Suárez-Alvarez , Yuri A. Roldán-Aragón , Rafael Romero-Martínez , Jorge H. Sahaza-Cardona , José Sifuentes-Osornio , Luis E. Soto-Ramírez , Gabriela R. Peña-Sandoval","doi":"10.1016/j.femsim.2005.05.017","DOIUrl":null,"url":null,"abstract":"<div><p>Three isolates of <span><em>Histoplasma capsulatum</em></span> were identified from mice lung, liver, and spleen inoculated with soil samples of the X hotel’s ornamental potted plants that had been fertilized with organic material known as compost. The presence of <em>H. capsulatum</em><span><span> in the original compost was detected using the dot-enzyme-linked immunosorbent assay. Nested-PCR, using a specific protein Hcp100 coding gene sequence, confirmed the fungal identification associated with an unusual </span>histoplasmosis outbreak in Acapulco. Although, diversity between the </span><em>H. capsulatum</em> isolate from the hotel and some clinical isolates from Guerrero (positive controls) was observed using random amplification of polymorphic DNA based-PCR, sequence analyses of <em>H-anti</em> and <em>ole</em> fragment genes revealed a high homology (92–99%) between them.</p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.05.017","citationCount":"40","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEMS immunology and medical microbiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0928824405001537","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 40
Abstract
Three isolates of Histoplasma capsulatum were identified from mice lung, liver, and spleen inoculated with soil samples of the X hotel’s ornamental potted plants that had been fertilized with organic material known as compost. The presence of H. capsulatum in the original compost was detected using the dot-enzyme-linked immunosorbent assay. Nested-PCR, using a specific protein Hcp100 coding gene sequence, confirmed the fungal identification associated with an unusual histoplasmosis outbreak in Acapulco. Although, diversity between the H. capsulatum isolate from the hotel and some clinical isolates from Guerrero (positive controls) was observed using random amplification of polymorphic DNA based-PCR, sequence analyses of H-anti and ole fragment genes revealed a high homology (92–99%) between them.