Identification of the infectious source of an unusual outbreak of histoplasmosis, in a hotel in Acapulco, state of Guerrero, Mexico

Maria Lucia Taylor , Guillermo M. Ruíz-Palacios , María del Rocío Reyes-Montes , Gabriela Rodríguez-Arellanes , Laura E. Carreto-Binaghi , Esperanza Duarte-Escalante , Aurora Hernández-Ramírez , Armando Pérez , Roberto O. Suárez-Alvarez , Yuri A. Roldán-Aragón , Rafael Romero-Martínez , Jorge H. Sahaza-Cardona , José Sifuentes-Osornio , Luis E. Soto-Ramírez , Gabriela R. Peña-Sandoval
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引用次数: 40

Abstract

Three isolates of Histoplasma capsulatum were identified from mice lung, liver, and spleen inoculated with soil samples of the X hotel’s ornamental potted plants that had been fertilized with organic material known as compost. The presence of H. capsulatum in the original compost was detected using the dot-enzyme-linked immunosorbent assay. Nested-PCR, using a specific protein Hcp100 coding gene sequence, confirmed the fungal identification associated with an unusual histoplasmosis outbreak in Acapulco. Although, diversity between the H. capsulatum isolate from the hotel and some clinical isolates from Guerrero (positive controls) was observed using random amplification of polymorphic DNA based-PCR, sequence analyses of H-anti and ole fragment genes revealed a high homology (92–99%) between them.

在墨西哥格雷罗州阿卡普尔科的一家酒店内,组织胞浆菌病异常暴发的传染源鉴定
用X酒店的观赏盆栽植物的土壤样本接种后,用有机材料堆肥施肥,从小鼠的肺部、肝脏和脾脏中分离出三株荚膜组织浆体。采用点酶联免疫吸附法检测原堆肥中荚膜荚膜菌的存在。利用特定蛋白Hcp100编码基因序列的巢式pcr证实了与阿卡普尔科不寻常的组织胞浆菌病暴发相关的真菌鉴定。虽然利用随机扩增的多态性DNA - pcr方法观察了从酒店分离的荚膜荚膜菌与格雷罗(阳性对照)临床分离的荚膜荚膜菌之间的差异,但对H-anti和ole片段基因的序列分析显示它们之间具有很高的同源性(92% - 99%)。
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