[Cultivation and identification of wheat-Elytrigia intermedium alien disomic addition lines].

Abstract

6 alien disomic addition lines (line0605; line0607, line0609, line0610, line0611, line0625), which were selected from 59 wheat-Elytrigia intermedium hybridization germ plasm lines, were identified by using the methods of morphology, powdery mildew resistance identifications, cytology and RAPD analysis. Morphogy results showed that they all had the better agronomic characters of their parents; Cytogic results showed that the chromosome configuration at PMC M I was 2n = 22 II; 209 random primers were used for RAPD amplification, result showed 5 of them amplified the specific bands of Elytrigia intermedium parents of 6 disomic addition lines, which could be used as RAPD markers for alien addition chromosomes. Results of powdery mildew resistance showed that line0605 was immune, line0607 was intermediately resistant, line 0610 and line0625 were highly resistant, and line0609 and line0611 were intermediately sensitive.