Biomimetic ion nanochannels for sensing umami substances

IF 12.9 1区医学 Q1 ENGINEERING, BIOMEDICAL

Biomaterials Pub Date : 2022-03-01 DOI:10.1016/j.biomaterials.2022.121418

Mingyang Li , Ninglong Zhang , Zhiyong Cui , Wenli Wang , Cunli Wang , Dongdong Wang , Minmin Li , Wenqi Lu , Guangyan Qing , Yuan Liu

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引用次数: 9

Abstract

Umami is one of the basic taste sensing, and represents the recognition of N-containing compounds capable of evaluating the nutritious contents of food. Although several sensors have been developed, the assessment of umami intensity remains challenging due to the limitations of sensor specificity, sensitivity, and performance stability. Here we present a biomimetic conical nanochannels system integrated with Venus flytrap (VFT) domain from human umami receptor T1R1 subunit to meet the concern. By taking advantage of sensitive transmembrane ionic flux change, the functional nanochannels could precisely distinguish umami substances from other tastants. Detailed mechanism analysis reveals that specific binding between T1R1 and umami substances triggers local conformation change and surface charge redistribution of the protein, which modulates the ionic current. This study initiates the application of nanochannel device in taste perception, which could help to disclose umami perception mechanism and screen new umami substances.

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传感鲜味物质的仿生离子纳米通道

鲜味是一种基本的味觉感知，是对含氮化合物的识别，能够评价食物的营养成分。虽然已经开发了几种传感器，但由于传感器的特异性、灵敏度和性能稳定性的限制，鲜味强度的评估仍然具有挑战性。为了解决这一问题，我们提出了一种基于人类鲜味受体T1R1亚基的捕蝇草(VFT)结构域的仿生锥形纳米通道系统。利用敏感的跨膜离子通量变化，功能纳米通道可以精确区分鲜味物质和其他味剂。详细的机理分析表明，T1R1与鲜味物质的特异性结合引发蛋白质局部构象改变和表面电荷重新分配，从而调节离子电流。本研究开创了纳米通道器件在味觉感知中的应用，有助于揭示鲜味感知机制和筛选新的鲜味物质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomaterials 工程技术-材料科学：生物材料

CiteScore

26.00

自引率

2.90%

发文量

565

审稿时长

46 days

期刊介绍： Biomaterials is an international journal covering the science and clinical application of biomaterials. A biomaterial is now defined as a substance that has been engineered to take a form which, alone or as part of a complex system, is used to direct, by control of interactions with components of living systems, the course of any therapeutic or diagnostic procedure. It is the aim of the journal to provide a peer-reviewed forum for the publication of original papers and authoritative review and opinion papers dealing with the most important issues facing the use of biomaterials in clinical practice. The scope of the journal covers the wide range of physical, biological and chemical sciences that underpin the design of biomaterials and the clinical disciplines in which they are used. These sciences include polymer synthesis and characterization, drug and gene vector design, the biology of the host response, immunology and toxicology and self assembly at the nanoscale. Clinical applications include the therapies of medical technology and regenerative medicine in all clinical disciplines, and diagnostic systems that reply on innovative contrast and sensing agents. The journal is relevant to areas such as cancer diagnosis and therapy, implantable devices, drug delivery systems, gene vectors, bionanotechnology and tissue engineering.