The metastatic potential of human pancreatic cell lines in the liver of nude mice correlates well with cathepsin B activity.

International journal of gastrointestinal cancer Pub Date : 2003-01-01 DOI:10.1385/IJGC:34:1:27

George N Tzanakakis, Andrew N Margioris, Aristidis M Tsatsakis, Michael P Vezeridis

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引用次数: 15

Abstract

Background: Cathepsin B, a lysosomal cysteine protease, has a major role in the mechanisms of tumor metastasis. The aim of the present work was to examine the correlation between cathepsin B activity and the metastatic potential of human pancreatic cancer.

Methods: The primary cell line COLO 357 and the derivative tumor cell lines FG, L3.1, L3.2, L3.3, L3.4, and L3.5, which are characterized by progressively increasing metastatic potential, were injected intrasplenically in the athymic mice. Cathepsin B activity, metastasis, and ultrastructural characteristics were assessed.

Results: An increased number of liver tumor nodules was observed with each subsequent intrasplenic inoculation (p = 0.001), associated with lymph node, splenic, and pancreatic involvement. Cathepsin B activity progressively increased (p = 0.001) and was strongly positively correlated with the metastatic potential. However, no correlation was found between the metastatic potential and ultrastructural characteristics.

Conclusions: These findings further support the central role of cathepsin B in metastasis in a combined in vitro/in vivo model.

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人胰腺细胞系在裸鼠肝脏中的转移潜能与组织蛋白酶B活性密切相关。

背景:组织蛋白酶B是一种溶酶体半胱氨酸蛋白酶，在肿瘤转移机制中起重要作用。本研究的目的是研究组织蛋白酶B活性与人胰腺癌转移潜能之间的关系。方法:将原代细胞系COLO 357和转移潜能逐渐增强的衍生肿瘤细胞系FG、L3.1、L3.2、L3.3、L3.4、L3.5分别注射于胸腺小鼠脾内。评估组织蛋白酶B活性、转移和超微结构特征。结果:每次脾内接种后，肝脏肿瘤结节数量增加(p = 0.001)，与淋巴结、脾和胰腺受累有关。组织蛋白酶B活性逐渐增加(p = 0.001)，并与转移潜能呈强正相关。然而，转移潜力与超微结构特征之间没有相关性。结论:在体外/体内联合模型中，这些发现进一步支持了组织蛋白酶B在转移中的核心作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International journal of gastrointestinal cancer

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