The anterograde and retrograde axonal transport of biotinylated dextran amine and biocytin in the nervous system of teleosts

Hao-Gang Xue, Chun-Ying Yang, Hironobu Ito
{"title":"The anterograde and retrograde axonal transport of biotinylated dextran amine and biocytin in the nervous system of teleosts","authors":"Hao-Gang Xue,&nbsp;Chun-Ying Yang,&nbsp;Hironobu Ito","doi":"10.1016/j.brainresprot.2004.03.001","DOIUrl":null,"url":null,"abstract":"<div><p><span>Biotinylated dextran amine (BDA) and </span>biocytin are well transported both retrogradely and anterogradely. Both tracers have stable molecular structure for long-term storage and examination, and their visualizations can be realized by simple histochemical reactions. Therefore, the BDA and biocytin are widely used in neuroanatomical studies as the tract-tracing markers. The results obtained by BDA and biocytin applications to various areas of the nervous system in teleosts were qualitatively identical, and the retrogradely and anterogradely labeled structures could be clearly identified with reference to the counter-staining. Iontophoretic injections or crystal insertions resulted in filling of cell bodies, dendrites and terminals in the core of injection side, revealing morphological details of the local and distant somata, dendritic arborizations and axonal terminals. However, biocytin exhibited superior to BDA in anterograde transport, and could label very thin axons, axonal collaterals and terminal ramifications. In contrast, retrograde transport of BDA was superior to that of biocytin, and resulted in more complete dendritic filling of retrograde labeled neurons including dendritic arborizations and spines.</p></div>","PeriodicalId":79477,"journal":{"name":"Brain research. Brain research protocols","volume":"13 2","pages":"Pages 106-114"},"PeriodicalIF":0.0000,"publicationDate":"2004-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.brainresprot.2004.03.001","citationCount":"23","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brain research. Brain research protocols","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1385299X04000364","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 23

Abstract

Biotinylated dextran amine (BDA) and biocytin are well transported both retrogradely and anterogradely. Both tracers have stable molecular structure for long-term storage and examination, and their visualizations can be realized by simple histochemical reactions. Therefore, the BDA and biocytin are widely used in neuroanatomical studies as the tract-tracing markers. The results obtained by BDA and biocytin applications to various areas of the nervous system in teleosts were qualitatively identical, and the retrogradely and anterogradely labeled structures could be clearly identified with reference to the counter-staining. Iontophoretic injections or crystal insertions resulted in filling of cell bodies, dendrites and terminals in the core of injection side, revealing morphological details of the local and distant somata, dendritic arborizations and axonal terminals. However, biocytin exhibited superior to BDA in anterograde transport, and could label very thin axons, axonal collaterals and terminal ramifications. In contrast, retrograde transport of BDA was superior to that of biocytin, and resulted in more complete dendritic filling of retrograde labeled neurons including dendritic arborizations and spines.

硬骨鱼神经系统中生物素化右旋糖酐胺和生物细胞素的顺行和逆行轴突转运
生物素化右旋糖酐胺(BDA)和生物细胞素都可以逆行和顺行运输。两种示踪剂均具有稳定的分子结构,便于长期储存和检测,且可通过简单的组织化学反应实现可视化。因此,BDA和生物细胞素被广泛应用于神经解剖学研究中。BDA和生物细胞素应用于硬骨鱼神经系统各区域的结果在质量上是一致的,并且通过反染色可以清楚地识别逆行和顺行标记的结构。离子渗透注射或晶体插入导致注射侧核心的细胞体、树突和终末填充,揭示了局部和远处体细胞、树突和轴突终末的形态学细节。然而,生物细胞素在顺行运输中表现优于BDA,可以标记非常薄的轴突、轴突侧枝和末端分支。相比之下,BDA的逆行转运优于生物细胞素,并导致逆行标记的神经元更完整的树突填充,包括树突和棘。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信