{"title":"[TLR4 mRNA expression and liver injury in LPS-induced mouse].","authors":"Xiao-wei Liu, Yu You, Fang-gen Lu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To determine the relationship between Toll-like receptor 4 (TLR4) mRNA expression and liver injury induced by lipopolysaccharide (LPS).</p><p><strong>Methods: </strong>Balb/c mice were sacrificed at the indicated time points after the intra-peritoneal LPS. The livers were stained with hematoxylin and erosin for histopathologic analysis. Simultaneously, the expression of TLR4mRNA was detected by RT-PCR.</p><p><strong>Results: </strong>A large number of inflammatory cells infiltrated into the portal areas and focal hepatocellular necrosis occurred 24 h after the intra-peritoneal LPS. The expression of TLR4mRNA in the liver was significantly depressed at 6 h and 12 h after the intra-peritoneal LPS, but recruited at 24 h.</p><p><strong>Conclusion: </strong>The signal transduction molecule TLR4 plays an important role in LPS-induced liver injury.</p>","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 3","pages":"217-20"},"PeriodicalIF":0.0000,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To determine the relationship between Toll-like receptor 4 (TLR4) mRNA expression and liver injury induced by lipopolysaccharide (LPS).
Methods: Balb/c mice were sacrificed at the indicated time points after the intra-peritoneal LPS. The livers were stained with hematoxylin and erosin for histopathologic analysis. Simultaneously, the expression of TLR4mRNA was detected by RT-PCR.
Results: A large number of inflammatory cells infiltrated into the portal areas and focal hepatocellular necrosis occurred 24 h after the intra-peritoneal LPS. The expression of TLR4mRNA in the liver was significantly depressed at 6 h and 12 h after the intra-peritoneal LPS, but recruited at 24 h.
Conclusion: The signal transduction molecule TLR4 plays an important role in LPS-induced liver injury.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
