Tempero-spatial dissociation between the expression of Fas and apoptosis after coronary occlusion.

Molecular pathology : MP Pub Date : 2003-12-01 DOI:10.1136/mp.56.6.362

Q Z Feng, T D Li, L X Wei, X Qiao, J Yi, L Wang, T S Yang

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引用次数: 8

Abstract

Aims: To explore the role of Fas in cardiomyocytic apoptosis induced by ischaemia through determining the histological relation between Fas expression and apoptosis in rat myocardium during ischaemia/infarction.

Methods: The myocardial ischaemia model was produced by ligating the left coronary artery in Sprague-Dawley rats. The rats were killed from 10 minutes to seven days after surgery. Apoptotic myocardial cells were detected by the in situ terminal deoxynucleotidyl transferase mediated nick end labelling method, and the expression of Fas by immunohistochemistry and western blotting.

Results: Cardiomyocytic apoptosis appeared from three to 36 hours after ischaemia. The expression of Fas could be detected by western blot from before surgery to seven days of ischaemia. Apoptosis and the expression of Fas in the cardiomyocytes appeared in different regions of the myocardium: apoptosis in the ischaemic region, Fas in the regions surrounding ischaemic myocardium.

Conclusion: These results suggest that there is a tempero-spatial dissociation between the expression of Fas and apoptosis after coronary occlusion. Fas might not directly regulate the apoptosis of cardiomyocytes induced by ischaemia.

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冠脉闭塞后Fas表达与细胞凋亡的时空分离。

目的:通过测定大鼠缺血/梗死心肌中Fas表达与细胞凋亡的组织学关系，探讨Fas在缺血心肌细胞凋亡中的作用。方法:结扎左冠状动脉造心肌缺血模型。这些老鼠在手术后10分钟到7天内被杀死。采用原位末端脱氧核苷酸转移酶介导的缺口末端标记法检测心肌细胞凋亡，免疫组织化学和免疫印迹法检测Fas表达。结果:缺血后3 ~ 36 h出现心肌细胞凋亡。从术前到缺血7 d, western blot均可检测Fas的表达。心肌细胞凋亡和Fas的表达出现在心肌的不同区域:缺血区凋亡，缺血心肌周围区Fas。结论:冠状动脉闭塞后Fas的表达与细胞凋亡存在时空分离关系。Fas可能不直接调控缺血引起的心肌细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Molecular pathology : MP

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