Relationship between endothelin 1 and nitric oxide system in the corpus luteum regression.

IF 2.9 4区医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 2003-11-01 DOI:10.1016/j.plefa.2003.07.002

T Tognetti, A Estevez, C G Luchetti, V Sander, A M Franchi, A B Motta

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Abstract

The present study was designed to investigate the relationship between the nitric oxide (NO) system and endothelin 1 (ET-1) in the mechanism of corpus luteum (CL) development and consequently regression in rats. We first evaluated basal ET-1 levels in ovarian tissue from rats with different stages of CL development. An increased ovarian ET-1 content was found during CL regression. In a dose-department response, ET-1 decreased progesterone (P4) and increased prostaglandin (PG) PGF2alpha production. By means of a competitive nitric oxide synthase (NOS) inhibitor: L-nitro arginine methyl ester (L-NAME) and a slow NO releasing: diethyl-aminetriamine (DETA-NONOate), we demonstrated that NO system could be the intermediary in the ET-1 diminishing P4 production. The Western blot analysis revealed an increase on iNOS while eNOS protein expression was diminished. We also found a diminution of total NOS activity after ET-1 treatment. These data suggest the existence of a functional relationship between ET-1 and NOS isoforms leading the regulation of CL functionally.

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黄体退化过程中内皮素1与一氧化氮系统的关系。

本研究旨在探讨一氧化氮(NO)系统与内皮素-1 (ET-1)在大鼠黄体(CL)发育及退化机制中的关系。我们首先评估了不同CL发展阶段大鼠卵巢组织中ET-1的基础水平。卵巢ET-1含量在CL回归中升高。在剂量部门反应中，ET-1降低孕酮(P4)并增加前列腺素(PG) pgf2 α的产生。通过竞争性一氧化氮合酶(NOS)抑制剂:l-硝基精氨酸甲酯(L-NAME)和缓慢释放NO的:二乙基胺三胺(delta - nonoate)，我们证明NO系统可能是ET-1减少P4产生的中介。Western blot分析显示，iNOS表达增加，eNOS蛋白表达减少。我们还发现ET-1治疗后总NOS活性降低。这些数据表明ET-1和NOS同工异构体之间存在功能关系，导致CL的功能调节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Prostaglandins, leukotrienes, and essential fatty acids 医学-内分泌学与代谢

CiteScore

6.40

自引率

6.70%

发文量

审稿时长

13.2 weeks

期刊介绍： The role of lipids, including essential fatty acids and their prostaglandin, leukotriene and other derivatives, is now evident in almost all areas of biomedical science. Cell membrane behaviour and cell signalling in all tissues are highly dependent on the lipid constituents of cells. Prostaglandins, Leukotrienes & Essential Fatty Acids aims to cover all aspects of the roles of lipids in cellular, organ and whole organism function, and places a particular emphasis on human studies. Papers concerning all medical specialties are published. Much of the material is particularly relevant to the development of novel treatments for disease.