Corrections of prooxidant-antioxidant homeostasis of organism under hypoxia of different genesis by yackton, a new pharmacological preparation.

Abstract

The aim of the present work was to study the antioxidant activity of the new derivative of succinic acid-succinate mono[(2-dimethyl-amino) ethyl ether] of succinic acid (yackton) under conditions of hypoxic and hemic hypoxia as well as to examine in vitro the antiradical activity of this preparation. Hypoxia in the rats was modelled by: (i) allowing the rats to breath a gas mixture with 7% O2 and 93% N2 for 30 minutes (hypoxic hypoxia), and (ii) injecting the rats sodium nitrite subcutaneously in a dose of 60 mg/kg body weight (hemic hypoxia). Yackton was injected intraperitoneally to both groups 30 minutes before the extreme influence in a dose of 140 mg/kg body weight. Then in homogenates and in post-mitochondrial fractions of liver, heart, lungs, brain we studied the content of secondary products of lipid peroxidation (LPO), activity of antioxidant enzymes superoxide dismutase (SOD) and catalase, and the activity of enzymes responsible for the maintenance of reduced glutathione (GSH) glutathione reductase (GR-ase) and glutathione peroxidase (GP-ase). In vitro studies were made on the antiradical activity of yackton in reaction with the stable radical diphenylpicrylhydrazine (DPPH) as well as on the reaction velocity of the preparation with DPPH, and its period of semi-transformation in a non-radical form. It was shown that yackton treatment before hypoxic and hemic hypoxia decreased lipid peroxidation (LPO) level and increased SOD activity. After the yackton injection the state of glutathione system was normalized in comparison with its state at hypoxic and hemic hypoxia. Yackton had no antiradical properties in vitro. We concluded that yackton promotes optimization of prooxidant-antioxidant homeostasis of the organism under the hemic and hypoxic hypoxia acting as antioxidant of a non-direct action.