Characterization of lipovitellenin components, and their relation to low-density liproprotein structure

J. Augustyniak, W.G. Martin, W.H. Cook
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引用次数: 14

Abstract

When aqueous solutions of the LDF of egg yolk and its fractions LDF1 and LDF2 were extracted with ethyl ether, all yielded five derived components having sedimentation rates of about 4 S, 7 S, 13 S, 15 S and 17 S. The first three of these were isolated and had molecular weights of 2.9·105, 11·105 and 13·105, and lipid contents of 50, 67 and 44%, respectively. These components were formed sequentially in the order 7 S → 4 S → 13 S and hence all contain the same proteins, although different combinations of polypeptide chains may be present. Apparently a reduction in lipid content below 50% causes an aggregation of 4-S particles to form 13 S and other rapidly sedimenting products. The sizes of the protein moieties, computed from the molecular weight and lipid content of the native fractions LDF1 and LDF2 and the derived products 7 S and 4 S, are in the order 8, 4, 2 and 1, respectively, with the smallest unit size estimated to be (1.7 ± 0.16) ·105. Evidently the size of the protein moiety decreases stepwise as the lipid content decreases down to 4 S and this behavior, plus aggregation at low lipid contents, yields detectable and separable components in both the natural and derived from of these lipoproteins.

脂卵黄素成分的表征及其与低密度脂蛋白结构的关系
用乙醚对蛋黄LDF水溶液及其部分LDF1和LDF2进行提取,得到5种衍生组分,沉淀速率分别为4 S、7 S、13 S、15 S和17 S,其中前3种分离得到,分子量分别为2.9·105、11·105和13·105,脂质含量分别为50%、67%和44%。这些成分是按7s→4s→13s顺序形成的,因此它们都含有相同的蛋白质,尽管可能存在不同的多肽链组合。显然,脂质含量降低到50%以下会导致4-S颗粒聚集形成13s和其他快速沉积产物。根据天然产物LDF1和LDF2以及衍生产物7s和4s的分子量和脂质含量计算,蛋白质片段的大小分别为8、4、2和1,最小单位大小估计为(1.7±0.16)·105。显然,当脂质含量降低到4 S时,蛋白质片段的大小逐渐减小,这种行为加上低脂质含量时的聚集,在这些脂蛋白中产生可检测和可分离的成分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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