The reaction of diazomethane with synthetic phosphatides and with insect lipid extracts

H.D. Crone
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引用次数: 14

Abstract

  • 1.

    1. The reaction of diazomethane with synthetic phosphatidylethanolamine, N-methylphosphatidylethanolamine, phosphatidylcholine and insect-lipid extracts was studied in order to find out if the reaction was specific to one type of phosphatide.

  • 2.

    2. The three synthetic phosphatides reacted in essentially the same manner, although with different rates of reaction, forming dimethyl esters of phosphatidic acids and a nitrogenous compound similar to neurine. The results with insect extracts gave evidence of the reaction of diazomethane with many natural phosphatides.

  • 3.

    3. N-methylation of the intact ethanolamine phosphatides occurred to a limited extent and was increased by the presnece of alcohols in the reaction medium.

  • 4.

    4. Techniques used included column chromatography on silicic acid, paper chromatography and infrared spectroscopy.

重氮甲烷与合成磷脂及昆虫脂质提取物的反应
1.1. 2.2研究了重氮甲烷与合成磷脂酰乙醇胺、n -甲基磷脂酰乙醇胺、磷脂酰胆碱和昆虫脂提取物的反应,以确定该反应是否对某一类磷脂具有特异性。这三种合成磷脂基本上以相同的方式反应,尽管反应速率不同,形成磷脂酸的二甲基酯和一种类似于神经氨酸的含氮化合物。用昆虫提取物的实验结果证明了重氮甲烷与许多天然磷脂的反应。完整的乙醇胺磷脂的n -甲基化在有限的程度上发生,并且由于反应介质中存在醇而增加。所使用的技术包括硅酸柱层析、纸层析和红外光谱。
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