Studies on the “independence” of nucleolar ribonucleic acid synthesis

Masami Muramatsu, James L. Hodnett, Harris Busch
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引用次数: 37

Abstract

Using [14C]orotic acid as a precursor, the time course of labeling of nuclear and nucleolar RNA of rat liver has been studied in vivo. RNA from both nuclear and nucleolar preparations was fractionated into two classes with the aid of phenol. Each class of RNA was found to have a relatively specific kinetic profile after administration of the tracer. Labeling of nucleolar RNA was as rapid as nuclear RNA indicating that the extranucleolar RNA could not be the precursor of the nucleolar RNA. Actinomycin D inhibited nucleolar-RNA synthesis to a much greater degree than the synthesis of extranucleolar RNA. These data, together with previous observations on the base compositions of nucleolar-RNA fractions and other types of experiments provide evidence that nucleolar-RNA synthesis largely proceeds “independently” of extranucleolar-RNA synthesis.

核仁核糖核酸合成的“独立性”研究
以[14C]乳香酸为前体,在体内研究了大鼠肝脏细胞核和核仁RNA标记的时间过程。在苯酚的帮助下,将核和核仁制备的RNA分成两类。在给药示踪剂后,发现每一类RNA具有相对特定的动力学特征。核仁RNA的标记速度与核RNA一样快,表明核外RNA不可能是核仁RNA的前体。放线菌素D对核仁RNA合成的抑制程度远高于对核外RNA合成的抑制程度。这些数据,连同先前对核仁rna组分的碱基组成的观察和其他类型的实验,提供了核仁rna合成在很大程度上“独立”于核外rna合成的证据。
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