The effect of metal ions on the binding of acridine orange to tobacco mosaic virus ribonucleic acid

K. Sivarama Sastry, Milton P. Gordon
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引用次数: 8

Abstract

TMV-RNA has been shown to form two types of complexes with acridine orange depending on whether the ratio of RNA-P: acridine orange is close to 1:1 (Complex I) or 20:1 (Complex II). Cr3+ and Cu2+ completely suppressed the formation of Complex I at levels around 5·10−4 M. Partial inhibition was observed with Ni2+, Co2+, Zn2+ and Mg2+ at 2.5·10−3–10·10−3 M, and with Hg+2 in the range 2.5·10−4–10·10−4 M. The inhibitory effectiveness of these metal ions followed the order Cr3+, Cu2+ > Ni2+, Co2+ > Zn2+ > Mg2+ > Hg2+. On the other hand, the reaction between TMV-RNA-P and acridine orange to form Complex II was modified by 1·10−5–10·10−5 M Cr3+, Cu2+, Ni2+, Mg2+ and Hg2+ in a different manner indicative of the formation of a new species with altered absorption and fluorescence characteristics. The new species, Complex III, consists of a RNA-acridine orange-metal ternary complex.

金属离子对吖啶橙与烟草花叶病毒核糖核酸结合的影响
TMV-RNA已被证明形成两种类型的配合物与吖啶橙的比例取决于RNA-P:吖啶橙接近1:1(复杂的)或20:1的三价铬,Cu2 +(复杂II)。完全压制的形成复杂的我水平在5·10−4 M .观察部分抑制Ni2 + Co2 + Zn2 +和Mg2 + 2.5 * 10−3 - 10 * 10−3 M和Hg范围2.5 + 2 * 10−十* 10−4 M .这些金属离子的抑制效力三价铬,跟着订单Cu2 +比;Ni2+, Co2+ >Zn2 +比;Mg2 +比;Hg2 +。另一方面,TMV-RNA-P与吖啶橙形成配合物II的反应被1·10−5 - 10·10−5 M Cr3+、Cu2+、Ni2+、Mg2+和Hg2+以不同的方式修饰,表明形成了一个新的物种,其吸收和荧光特性发生了改变。新发现的络合物III由rna -吖啶橙-金属三元络合物组成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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