Studies on animal hemoglobins II. The influence of inorganic phosphate on the physico-chemical and physiological properties of the hemoglobin of the adult chicken

Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects Pub Date : 1964-09-25 DOI:10.1016/0926-6577(64)90190-1

T.H.J. Huisman, J.M.Schillhorn Van Veen, A.M. Dozy, C.M. Nechtman

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引用次数: 20

Abstract

The equilibrium of dilute and concentrated adult chicken red blood cell hemolysates with O₂ has been studied using potassium phosphate buffers and NaCl solutions of different molarities. The results indicate an increase in O₂ affinity with increase in ionic strength. The same phenomenon was observed for the isolated minor hemoglobin component; the isolated major hemoglobin component showed a rather high O₂ affinity at low ionic strength, which decreased with increase in molarity. The minor hemoglobin fraction exhibited an ability to form rather stable complexes with phosphate ions; such complexes possessed a high O₂ affinity at low ionic strength. The data also indicated that the two hemoglobin components in a hemolysate interacted with each other. This extra-molecular interaction interfered with the physiologic properties of the hemolysate. No physico-chemical interaction could be detected, as was demonstrated by the determination of the molecular weight and by viscosity measurements.

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动物血红蛋白的研究2。无机磷酸盐对成年鸡血红蛋白理化生理特性的影响

用磷酸钾缓冲液和不同摩尔浓度的NaCl溶液研究了成年鸡红细胞溶血液的稀释和浓缩平衡。结果表明，随着离子强度的增加，氧的亲和力也随之增加。在分离的少量血红蛋白成分中也观察到同样的现象;分离的主要血红蛋白组分在低离子强度下表现出较高的氧亲和力，随着摩尔浓度的增加而降低。次要血红蛋白部分表现出与磷酸盐离子形成相当稳定的复合物的能力;这种配合物在低离子强度下具有高氧亲和力。数据还表明，溶血液中的两种血红蛋白成分相互作用。这种分子外相互作用干扰了溶血物的生理特性。没有检测到物理化学相互作用，正如分子量测定和粘度测量所证明的那样。

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects

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