Studies of animal hemoglobins III. The possible role of intercellular inorganic phosphate on the oxygen equilibrium of the hemoglobin in the developing chicken

T.H.J. Huisman, J.M.Schillhorn Van Veen
{"title":"Studies of animal hemoglobins III. The possible role of intercellular inorganic phosphate on the oxygen equilibrium of the hemoglobin in the developing chicken","authors":"T.H.J. Huisman,&nbsp;J.M.Schillhorn Van Veen","doi":"10.1016/0926-6577(64)90191-3","DOIUrl":null,"url":null,"abstract":"<div><p>The hemoglobin of red cell hemolysates of chicken embryos and chickens after hatching have been reinvestigated using starch-gel electrophoresis and O<sub>2</sub> equilibrium measurements. Thee hemoglobin components were found, namely the major and minor hemoglobin fractions also present in the adult chicken red cell hemolysates and a slow-moving fraction, which disappeared gradually from the blood after hatching. The electrophoretic mobility of the minor hemoglobin fraction in embryonic red cell hemolysates was slower than that of the fractions observed in 1-day-old chick and in adult chicken hemolysates, and was the same as that produced by preincubation of these hemolysates with 0.2 M phospahte buffers. It was also observed that the erythrocytic inorganic phosphate level was several times higher in the newborn chicken than in the adult fowl; the decrease in phosphate level after hatching coincides with the decrease in O<sub>2</sub> affinity of total blood samples as observed by others. The equilibrium of the total red cell hemolysate of a 1-day-old chicken with O<sub>2</sub> showed a higher O<sub>2</sub> affinity at low phosphate molarity, which decreased with increased phosphate concentration in a similar ways as observed for adult red cell hemolysates being pre-exposed to phosphate. The possible role of the phospahte content of the red cell in the regulation of the O<sub>2</sub> affinity of the chicken hemoglobin during development has been discussed.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1964-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90191-3","citationCount":"25","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926657764901913","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 25

Abstract

The hemoglobin of red cell hemolysates of chicken embryos and chickens after hatching have been reinvestigated using starch-gel electrophoresis and O2 equilibrium measurements. Thee hemoglobin components were found, namely the major and minor hemoglobin fractions also present in the adult chicken red cell hemolysates and a slow-moving fraction, which disappeared gradually from the blood after hatching. The electrophoretic mobility of the minor hemoglobin fraction in embryonic red cell hemolysates was slower than that of the fractions observed in 1-day-old chick and in adult chicken hemolysates, and was the same as that produced by preincubation of these hemolysates with 0.2 M phospahte buffers. It was also observed that the erythrocytic inorganic phosphate level was several times higher in the newborn chicken than in the adult fowl; the decrease in phosphate level after hatching coincides with the decrease in O2 affinity of total blood samples as observed by others. The equilibrium of the total red cell hemolysate of a 1-day-old chicken with O2 showed a higher O2 affinity at low phosphate molarity, which decreased with increased phosphate concentration in a similar ways as observed for adult red cell hemolysates being pre-exposed to phosphate. The possible role of the phospahte content of the red cell in the regulation of the O2 affinity of the chicken hemoglobin during development has been discussed.

动物血红蛋白的研究III。细胞间无机磷酸盐对发育中的鸡血红蛋白氧平衡的可能作用
用淀粉凝胶电泳法和氧平衡法对鸡胚和鸡孵化后红细胞溶血物的血红蛋白进行了研究。在成年鸡红细胞溶血液中发现了三种血红蛋白成分,即主要血红蛋白和次要血红蛋白,以及缓慢移动的血红蛋白,在孵化后逐渐从血液中消失。胚胎红细胞溶血液中少量血红蛋白部分的电泳迁移率低于1日龄鸡和成年鸡溶血液中所观察到的部分,与用0.2 M磷酸盐缓冲液预孵育这些溶血液所产生的电泳迁移率相同。还观察到新生鸡红细胞无机磷酸盐水平比成年鸡高几倍;孵化后磷酸盐水平的下降与其他人观察到的总血液样品氧亲和力的下降相一致。1日龄鸡总红细胞溶血液与O2的平衡在低磷酸盐摩尔浓度下表现出较高的O2亲和力,随着磷酸盐浓度的增加而降低,与预暴露于磷酸盐的成体红细胞溶血液相似。本文讨论了红细胞中磷酸盐含量在鸡血红蛋白发育过程中对氧亲和力的调节中可能起的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信