Ribosomes and polyribosomes in Brassica pekinensis

M.F. Clark, R.E.F. Matthews, R.K. Ralph
{"title":"Ribosomes and polyribosomes in Brassica pekinensis","authors":"M.F. Clark,&nbsp;R.E.F. Matthews,&nbsp;R.K. Ralph","doi":"10.1016/0926-6550(64)90253-1","DOIUrl":null,"url":null,"abstract":"<div><p>Leaves of <em>Brassica pekinensis</em> plants contain 83-S ribosomes which occur free in the cytoplasm rather than in chloroplasts. A 68-S class of ribosomes present in 20–35% the amount of the 83-S, occurs largely if not entirely in chloroplasts. Both classes may occur as polyribosomal aggregates which can be temporarily preserved in leaf extracts using polyvinyl sulphate as a nuclease inhibitor. The major environmental influence on polyribosome levels in leaves is a diurnal cycle in which light is the predominant factor. Polyribosomes decrease in amount during the night and reach their lowest level before dawn. 2 or 3 h after sunrise 80–90% of the 83-S ribosomes may be in polyribosomes. If the natural dark period is extended for 2–3 h no polyribosomes can be detected. When such plants are exposed to sunlight a rise in polyribosomes and a drop in 83-S ribosomes can be detected within 4 min. This light-dependent increase in polyribosomes appears to be associated, at least in part, with the synthesis of new RNA.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 2","pages":"Pages 289-304"},"PeriodicalIF":0.0000,"publicationDate":"1964-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90253-1","citationCount":"88","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926655064902531","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 88

Abstract

Leaves of Brassica pekinensis plants contain 83-S ribosomes which occur free in the cytoplasm rather than in chloroplasts. A 68-S class of ribosomes present in 20–35% the amount of the 83-S, occurs largely if not entirely in chloroplasts. Both classes may occur as polyribosomal aggregates which can be temporarily preserved in leaf extracts using polyvinyl sulphate as a nuclease inhibitor. The major environmental influence on polyribosome levels in leaves is a diurnal cycle in which light is the predominant factor. Polyribosomes decrease in amount during the night and reach their lowest level before dawn. 2 or 3 h after sunrise 80–90% of the 83-S ribosomes may be in polyribosomes. If the natural dark period is extended for 2–3 h no polyribosomes can be detected. When such plants are exposed to sunlight a rise in polyribosomes and a drop in 83-S ribosomes can be detected within 4 min. This light-dependent increase in polyribosomes appears to be associated, at least in part, with the synthesis of new RNA.

北京芸苔的核糖体和多核糖体
北京芸苔叶片中含有游离于细胞质而非叶绿体中的83-S核糖体。68-S类核糖体的数量是83-S核糖体的20-35%,即使不是全部也大部分存在于叶绿体中。这两类都可能以多核糖体聚集体的形式出现,这些聚核体聚集体可以用聚乙烯醇硫酸盐作为核酸酶抑制剂暂时保存在叶片提取物中。环境对叶片中多核糖体水平的主要影响是昼夜循环,其中光是主要因素。夜间多核糖体的数量减少,黎明前达到最低水平。日出后2或3小时,80-90%的83-S核糖体可能在多核糖体中。如果自然暗期延长2-3小时,则无法检测到多核糖体。当这些植物暴露在阳光下时,可以在4分钟内检测到多核糖体的增加和83-S核糖体的下降。这种依赖于光的多核糖体的增加似乎与新RNA的合成有关,至少部分相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信