Magdalena Bamburowicz-Klimkowska , Monika Ruzycka-Ayoush , Andrzej Cieszanowski , Wojciech Szeszkowski , Malgorzata Bialek , Anna Malkowska , Ireneusz P. Grudzinski
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引用次数: 0
Abstract
Background
Non-alcoholic fatty liver disease (NAFLD) is considered to be the leading cause of liver pathologies worldwide. Accurate diagnosis and staging of NAFLD is of utmost prognostic importance. Herein, we propose the application of multiparametric MRI in whole-body fat imaging that may serve in obesity and NAFLD diagnosis as we established based on an experimental preclinical model of high-calorie diet-induced NAFLD rats.
Methods and results
Sprague Dawley male rats randomly divided into control and high-fat diet (HFD) groups to develop NAFLD were used in the experiments. After 12 weeks of the feeding the animals were subjected to MRI modalities based on the Dixon mode and DWI following T2-weighted imaging. Moreover, serum TAG, liver histopathological examination and liver fatty acids analysis (GC-MS) were also performed.
The qualitative analysis of DWI images revelated the decrease of signals in the liver of rats subjected to HFD. The statistical analysis of signals from the water- and fat-separated voxels on Dixon images also showed the increase of the fat tissue along with the decrease of water molecules in the liver parenchyma of obese animals. The quantitative analysis of Dixon images allowed to estimate the statistically significant changes of whole-body fat profiles in both normal and obese rats. Histological analysis of the liver tissues, serum TAG and fatty acids profile in the livers confirmed the changes in the fat profile as demonstrated in MRI studies.
Conclusions
The MRI-based modalities such as DWI and Dixon method provide both qualitative and quantitative data on the whole-body fat distribution and adipose tissues in the liver parenchyma of obese rats. The results show that MRI is a promising and reliable method and has potential to be used as a non-invasive translational biomarker in NAFLD.
期刊介绍：
Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications.
Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.
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