Molecular structure modification of ovalbumin through controlled glycosylation with dextran for its emulsibility improvement

IF 8.5 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2022-01-01 DOI:10.1016/j.ijbiomac.2021.11.130

Yimei Zheng , Yu Chang , Biying Luo , Hui Teng , Lei Chen

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引用次数: 25

Abstract

Ovalbumin (OVA) is a high nutritious protein, but the poor emulsibility limited its application. The present study glycosylated OVA with dextran (Dex) by controlled wetheating (60–90 °C for 3 h). Temperature was an inductive factor for glycosylation degree (DG and browning intensity), and higher temperature could accelerate the reaction. Variations in molecular structure of OVA were analyzed by SDS-PAGE, FTIR, fluorescence spectroscopy and UV spectroscopy, which verified successes in the generation of glycoconjugate with more flexible structure. Emulsifying activity index (EAI) and emulsion stability index (ESI) for the emulsion of OVA-Dex glycoconjugates were significantly enhanced with the increasing of glycosylation temperature. Moreover, confocal laser scanning results revealed that the emulsion exhibited smaller size and more uniform distribution, and slower transmission profiles were checked by LUMiSizer centrifugal analysis as well, confirming the emulsibility improvement of OVA. Thus, controlled glycosylation reaction is an available method to improve the emulsifying properties of OVA.

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葡聚糖控制糖基化对卵清蛋白分子结构的修饰以改善其乳化性

卵清蛋白是一种高营养蛋白，但其乳化性差限制了其应用。本研究通过控制温度(60-90℃，3 h)将OVA与葡聚糖(Dex)糖基化。温度是糖基化程度(DG和褐变强度)的诱导因素，较高的温度可以加速反应。通过SDS-PAGE、FTIR、荧光光谱和UV光谱分析了OVA分子结构的变化，验证了生成结构更灵活的糖缀合物的成功。OVA-Dex糖缀合物的乳化活性指数(EAI)和乳液稳定性指数(ESI)随着糖基化温度的升高而显著增强。此外，激光共聚焦扫描结果显示，乳化液尺寸更小，分布更均匀，LUMiSizer离心分析也检查了较慢的透射曲线，证实了OVA的乳化性改善。因此，控制糖基化反应是改善OVA乳化性能的一种有效方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.