Ag/ZnO/Au 3D hybrid structured reusable SERS substrate as highly sensitive platform for DNA detection

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2019-01-15 DOI:10.1016/j.snb.2018.09.085

Anil Kumar Pal , Sudhakar Pagal , Kenchappa Prashanth , Goutam Kumar Chandra , Siva Umapathy , D. Bharathi Mohan

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引用次数: 69

Abstract

Ultra-sensitive hybrid Silver/Zinc oxide/Gold (Ag/ZnO/Au) structure based three dimensional (3D) surface enhanced Raman scattering (SERS) substrates have been prepared by three step fabrication process using thermal evaporation, hydrothermal growth, and sputtering techniques. The size and inter-particle (IP) gap of decorated Au nanoparticles (NPs) on ZnO nanorods (NRs) in the sub-nanometer range have been achieved through varying the sputtering time of Au. The superhydrophobic nature, the formation of the Schottky barrier at ZnO/Au interface and the broad optical absorption spectrum facilitated towards higher SERS activity of Ag/ZnO/Au hybrid structures. The higher SERS activity of 3D SERS substrate as compared with two dimensional (2D) SERS substrate has been studied. The good SERS signal reproducibility of 3D hybrid structures have been explored through Raman mapping. Higher SERS enhancement factor (EF) of 1 × 10¹⁰ has been achieved with a limit of detection (LOD) up to 10⁻¹⁶ M and 10 ng/μL for Rhodamine-6 G (Rh6G) and lambda DNA (λ-DNA), respectively. The degradation of Rh6G and λ-DNA molecules have been studied through photocatalytic degradation process to explore the reusability of the SERS substrates up to 10 and 4 times, respectively, with maintaining good SERS signal reproducibility. This metal/semiconductor/metal hybrid structure based SERS substrate with reusable capability indicates potential application towards biosensor for the detection of biologically important molecules at very low concentration level.

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Ag/ZnO/Au三维复合结构可重复使用SERS底物作为DNA检测的高灵敏度平台

采用热蒸发、水热生长和溅射三步法制备了超灵敏的银/氧化锌/金(Ag/ZnO/Au)杂化结构的三维表面增强拉曼散射(SERS)衬底。通过改变Au溅射时间，获得了修饰后的Au纳米颗粒在ZnO纳米棒上亚纳米范围内的尺寸和颗粒间间隙。Ag/ZnO/Au杂化结构的超疏水性、ZnO/Au界面上Schottky势垒的形成以及广泛的光学吸收光谱有利于提高SERS活性。研究了三维SERS底物比二维SERS底物具有更高的SERS活性。利用拉曼映射技术探索了三维杂化结构良好的SERS信号再现性。对罗丹明-6 G (Rh6G)和λ DNA (λ-DNA)的检测限(LOD)分别达到10−16 M和10 ng/μL, SERS增强因子(EF)达到1 × 1010。通过光催化降解过程研究了Rh6G和λ-DNA分子的降解，探索了SERS底物分别可重复使用10次和4次，并保持了良好的SERS信号再现性。这种基于金属/半导体/金属混合结构的SERS衬底具有可重复使用的能力，在生物传感器中具有潜在的应用前景，可用于检测极低浓度水平的生物重要分子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.