Growth and sporulation of some pathogenic fungi in the presence of grapefruit extract.

Leszek B Orlikowski, Cz Skrzypczak, A Jaworska-Marosz
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Abstract

Development of Fusarium oxysporum f. sp. cyclaminis, F. oxysporum f. sp. dianthi, Botrytis cinerea and B. elliptica in the presence of grapefruit extract (GE) was evaluated. Amendment of potato-dextrose agar with 40 micrograms of GE/cm3 inhibited linear growth of tested species at least in 50%. Addition of 40 micrograms of GE/cm3 of medium inhibited spore germination of F. oxysporum f.sp. cyclaminis about 34% whereas germ tube growth was suppressed in 87%. In case of Botrytis species, B. cinerea spores were more susceptible on GE than B. elliptica. They did not germinate, however, at 200 micrograms of GE/cm3. Drenching of peat, artificially infested with F. oxysporum f. sp. dianthi, with GE at conc. 165 micrograms/cm3 resulted in drastical decrease of colony forming unit numbers and this suppressive effect was observed at least 35 days.

葡萄柚提取物对病原菌生长和产孢的影响。
研究了柚子提取物(GE)对环绿镰刀菌(Fusarium oxysporum f. sp. cyclaminis)、尖孢镰刀菌(f.s oxysporum f.sp . dianthi)、灰霉病菌(Botrytis cinerea)和椭圆镰刀菌(b.s elliptica)的影响。用40微克GE/cm3的浓度对马铃薯葡萄糖琼脂进行修饰,至少抑制了50%的被试物种的线性生长。添加40微克GE/cm3的培养基对尖孢菌孢子萌发有抑制作用。胞霉素抑制率为34%,而胚管生长抑制率为87%。在葡萄孢中,灰孢杆菌孢子对GE的敏感性高于椭圆孢杆菌。然而,在200微克GE/cm3的环境下,它们没有发芽。泥炭的浸水,人工侵染尖孢镰刀菌。165微克/立方厘米可显著降低菌落形成单位数,且这种抑制作用至少持续35天。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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