Capillary electrophoresis as a method for determining the hydrolysis rate constant of raffinose.

Abstract

Capillary electrophoresis was first employed for the determination of the hydrolysis rate constant of trisaccharide raffinose. Raffinose hydrolyzes in acidic medium at different temperatures; the hydrolysis products, including melibiose and fructose, were determined quantitatively. The concentration change in hydrolysis products over time provided useful information for calculating the hydrolysis constant of raffinose. The temperature dependence of the hydrolysis rate constant was used to calculate raffinose hydrolysis activation energy, which corresponds to the literature value.