Capillary electrophoretic separation by double-strand polyaniline-coate capillaries of the advanced glycation endproducts formed from N-alpha-acetyl-L-lysine with reducing sugars.
Paula F G de Sa, Christina Robb, Elmo Resende, Patrick McCarthy, Sze C Yang, Phyllis R Brown, Joel A Dain
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Abstract
Capillary electrophoresis using a capillary coated with a double-strand coating of polyaniline:poly(methyacrylate-co-acrylic acid) (PAN:P[MA-AAI) was used to separate advanced glycation endproducts (AGEs) formed at 37 degrees C from model systems containing either glucose (Glc), fructose (Fru), or glyceraldehyde (GA) and N-alpha-acetyl-L-lysine (NALys). The presence of the P(MA-AA) as a second strand in the polymer allows the maintenance of the conductive state of the PAN at a wide pH range. Effects of buffer pH and coating concentration on the electroosmotic flow (EOF) were investigated. More AGE species can be detected for the GA/NALys mixtures using this coated capillary than upon an uncoated capillary. The coating procedure is simple and the stability of the coated capillary is good.
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