Spectral karyotyping of mouse cell line WMP2.

G Liu, W Lu, S Bremer, H Hameister, B Schreiner, M Hughes, H H Heng
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引用次数: 15

Abstract

We have evaluated the mouse cell line WMP2 using both GTG-banding analysis and spectral karyotyping to verify the reliability of using this established cell line derived from WMP/WMP mice. The WMP cell lines contain easily identifiable metacentric fusion chromosomes and are used extensively for gene mapping. Because of karyotypical changes in the WMP1 cell line, WMP2 was examined. Our results demonstrate that WMP2 is stable during culture, and the karyotype is simple and easy to use. Based on the findings discussed in this paper, we recommend the use of the WMP2 cell line for future prospective gene mapping in the mouse.

小鼠WMP2细胞系的光谱核型分析。
我们对小鼠细胞系WMP2进行了gtg -band分析和光谱核型分析,以验证使用该来源于WMP/WMP小鼠的已建立细胞系的可靠性。WMP细胞系含有易识别的异心融合染色体,广泛用于基因定位。由于WMP1细胞系的核型改变,我们检测了WMP2。结果表明,WMP2在培养过程中稳定,核型简单,易于使用。基于本文讨论的结果,我们建议使用WMP2细胞系进行未来小鼠基因定位。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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