Assignment of TRA1 encoding ppk98 to pig chromosome 5 by fluorescent in situ hybridization and confirmation by somatic cell hybrid analysis.

Abstract

Porcine protein kinase 98 (ppk98) is a stress-inducible glycoprotein that is known to be constitutively and ubiquitously expressed in the endoplasmic reticulum of mammalian cells (Dechert et al., 1994). The genomic DNA sequence of the ppk98 gene has been determined by Konig et al. (1997). Sequence analyses revealed domains that are highly conserved with the human tumor rejection antigen (TRA1) or glucose-regulated protein (GRP94) genes. These findings suggest that these proteins are either identical or represent a family of closely related proteins (Konig et al., 1997). The human and mouse TRA1 genes have been located to human (HSA) chromosome 12 and mouse (MMU) chromosome 10, but, as yet, the porcine ppk98 gene (TRA1) has not been mapped. We report the localization of the pig ppk98 gene (TRA1) to porcine chromosome 5 by fluorescent in situ hybridization and somatic cell hybrid panel. This assignment supports the hypothesis that the ppk98 gene is the porcine homologue of the human TRA1 gene. Materials and methods