Assignment of the murine def-3 gene (Rbm6) to chromosome 9F1-F2 and its pseudogenes Rbm6-ps1 and Rbm6-ps2 to chromosome 1 by in situ hybridisation.

Abstract

The mouse def-3 gene (Rbm6), which defines a novel family of RNA binding proteins, was isolated from differentiating myeloid progenitor cells (Hotfilder et al., 1999). The human homologue was identified from the human 3p21.3 small cell lung cancer (SCLC) deletion region (DEF-3: Drabkin et al., 1999; RBM6: Timmer et al., 1999) and as a target for autoantibodies in SCLC (NY-LU-12: Gure et al., 1998). Here, we report isolating murine genomic clones, representing (1) the def-3 locus (Rbm6), (2) a processed pseudogene Rbm6-ps1 and (3) an apparently non-processed pseudogene Rbm6-ps2 (Table 1). Using FISH, we assigned murine Rbm6 to chromosome 9F1–F2 (Fig. 1A). In addition, the two pseudogenes both map to chromosome 1, in regions A3–A5 (Rbm6-ps1) and G (Rbm6-ps2) (Fig. 1B).